[大鼠耳蜗中L型电压门控性Ca2+通道的表达模式]
[The expression pattern of L-type voltage-gated Ca2+ channel in rat cochlea].
作者信息
Shen Weidong, Hu Yinyan, Yang Weiyan, Han Dongyi
机构信息
Department of Otolaryngology-Head and Neck Surgery, PLA General Hospital, Beijing, 100853, China.
出版信息
Lin Chuang Er Bi Yan Hou Ke Za Zhi. 2004 Oct;18(10):613-5.
OBJECTIVE
To investigate the molecular basis of the voltage-gated Ca2+ channel (VGCC) which controls the afferent synaptic neurotransmitters release in inner hair cell and their cellular expression.
METHOD
In this study we used different and complementary approaches to determine which types of 4 subunits of VGCC were expressed in the organ of Corti.
RESULT
RT-PCR analysis showed that alpha1C and alpha1D were present in the organ of Corti total RNA extract, and the corresponding antisense riboprobes could detect the expression of alpha1C and alpha1D in IHCs and OHCs.
CONCLUSION
The voltage-gated Ca2+ channel expressed in sensory cell in organ of Corti are alpha1C and alpha1D subtypes.
目的
研究控制内毛细胞传入突触神经递质释放的电压门控钙离子通道（VGCC）的分子基础及其细胞表达。
方法
在本研究中，我们采用了不同且互补的方法来确定VGCC的4种亚基类型中哪些在柯蒂氏器中表达。
结果
逆转录-聚合酶链反应（RT-PCR）分析表明，α1C和α1D存在于柯蒂氏器总RNA提取物中，相应的反义核糖探针可检测到α1C和α1D在内毛细胞（IHCs）和外毛细胞（OHCs）中的表达。
结论
柯蒂氏器感觉细胞中表达的电压门控钙离子通道是α1C和α1D亚型。
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