Xie Dinghua, Hu Peng, Xiao Zi'an, Wu Weijing, Chen Yong, Xia Kun
Department of Otolaryngology and Head & Neck Surgery of the Second Xiangya Hospital and Institute of Otology, Changsha, People's Republic of China.
Acta Otolaryngol. 2007 Jan;127(1):8-12. doi: 10.1080/00016480600627927.
The results show that alpha1D, alpha1E, alpha2/delta, beta1, and beta3 subunits are expressed in spiral ganglion cells (SGCs), and the coexpression of alpha1D and alpha1E suggests the presence of L-type and R-type calcium channels in mammalian SGCs.
To investigate the types of subunits of voltage-gated calcium channels in SGCs of the mouse.
SGCs were isolated from cochleae of neonatal mice and cultured for 24 h. Total RNA was extracted from cultured cells. After reverse transcription, the resulting cDNA was amplified by PCR with primers targeted to nucleotide sequences corresponding to seven different calcium channel subunits. The types of calcium channel subunits were identified by PCR analysis and nucleotide sequencing.
RT-PCR showed the strong and consistent amplification of alpha1D, alpha1E, alpha2/delta, beta1, and beta3 subunits from the mRNA of SGCs, and nucleotide sequencing confirmed the identity of mouse cochlear subunit cDNAs.
结果表明，α1D、α1E、α2/δ、β1和β3亚基在螺旋神经节细胞（SGCs）中表达，α1D和α1E的共表达提示哺乳动物SGCs中存在L型和R型钙通道。
研究小鼠SGCs中电压门控钙通道亚基的类型。
从小鼠新生儿耳蜗中分离出SGCs并培养24小时。从培养的细胞中提取总RNA。反转录后，用针对对应于七种不同钙通道亚基核苷酸序列的引物通过PCR扩增所得的cDNA。通过PCR分析和核苷酸测序鉴定钙通道亚基的类型。
RT-PCR显示从SGCs的mRNA中强烈且一致地扩增出α1D、α1E、α2/δ、β1和β3亚基，核苷酸测序证实了小鼠耳蜗亚基cDNA的身份。
