水翁对脂质过氧化及神经元细胞损伤的保护作用
[Protective effects of Cleistocalyx operculatus on lipid peroxidation and trauma of neuronal cells].
作者信息
Lu Yan-hua, Du Chang-bin, Wu Zi-bin, Ye Chun-lin, Liu Jian-wen, Wei Dong-zhi
机构信息
State Key Laboratory of Bioreactor Engineering, Institute of Biochemistry, East China University of Science and Technology, Shanghai 200237, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2003 Oct;28(10):964-6.
OBJECTIVE
To observe the protective effects of Cleistocalyx operculatus on lipid peroxidation in rat liver microsomes and on the trauma of PC12 cells induced by H2O2.
METHOD
The mouse liver homogenate lipid peroxidation assay and PC12 Cell culture and Cell viability (MTT assay) were applied.
RESULT
Cleistocalyx operculatus showed strong protective effects on lipid peroxidation in rat liver microsomes in a dose-dependent manner and exhibited potent protective effects on the trauma of PC12 cells induced by H2O2 (200 micromol x L(-1)) when the concentration reached 1.00 g x L(-1).
CONCLUSION
Cleistocalyx operculatus may be used as antioxidant to prevent or delay the pathogenesis of neural cell diseases.
目的
观察水翁对大鼠肝微粒体脂质过氧化及H2O2诱导的PC12细胞损伤的保护作用。
方法
采用小鼠肝匀浆脂质过氧化测定法以及PC12细胞培养和细胞活力（MTT法）。
结果
水翁对大鼠肝微粒体脂质过氧化具有较强的保护作用，呈剂量依赖性，当浓度达到1.00 g·L-1时，对H2O2（200 μmol·L-1）诱导的PC12细胞损伤具有显著的保护作用。
结论
水翁可作为抗氧化剂预防或延缓神经细胞疾病的发病机制。
Zotero 插件