Yeo M, Rha S Y, Jeung H C, Shen X H, Yang S H, An S W, Roh J K, Chung H C
Cancer Metastasis Research Center, Yonsei Cancer Center, Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
FEBS Lett. 2005 Jan 3;579(1):127-32. doi: 10.1016/j.febslet.2004.11.058.
Even if template sequence of hTR played an essential role in telomere binding, a 326 nucleotide fragment of hTR containing template, pseudoknot, and CR4-5 domains is critical for both binding with telomeric DNA and reconstitution of telomerase activity. A functional study with antisense oligonucleotides suggested that targeted disruption of the template region efficiently abrogated both telomeric DNA binding and telomerase activity, whereas disruption of the CR4-5 region induced only loss of telomerase activity. hTR interacts with telomeric DNA via structural region composed of the template, pseudoknot, and CR4-5 domains, however, each structural domain plays a distinct role in telomere binding and telomerase activity reconstitution.
即使hTR的模板序列在端粒结合中起关键作用，但包含模板、假结和CR4-5结构域的326个核苷酸的hTR片段对于与端粒DNA结合以及端粒酶活性的重建都至关重要。一项使用反义寡核苷酸的功能研究表明，靶向破坏模板区域可有效消除端粒DNA结合和端粒酶活性，而破坏CR4-5区域仅导致端粒酶活性丧失。hTR通过由模板、假结和CR4-5结构域组成的结构区域与端粒DNA相互作用，然而，每个结构域在端粒结合和端粒酶活性重建中都发挥着独特的作用。
