Forcic Dubravko, Cakanic Karmen Branovic, Ivancic Jelena, Jug Renata, Barut Milos, Strancar Ales, Mazuran Renata
Institute of Immunology Inc., Rockefeller Street 10, 10000 Zagreb, Croatia.
Anal Biochem. 2005 Jan 15;336(2):273-8. doi: 10.1016/j.ab.2004.10.018.
Analysis of crude samples from biotechnological processes is often required to demonstrate that residual host cell impurities are reduced or eliminated during purification. Current knowledge suggests that a continuous-cell-line DNA can be considered a cellular contaminant rather than a significant risk factor requiring removal to extremely low levels. Anion-exchange chromatography is one of the most important methods used in the downstream processing and analysis of different biomolecules. In this article, an application using Convective Interaction Media monolithic columns to improve the detection of residual cellular DNA is described.
生物技术过程中粗样品的分析常常需要证明在纯化过程中残留的宿主细胞杂质已减少或消除。目前的认知表明，连续细胞系DNA可被视为一种细胞污染物，而非需要去除至极低水平的重大风险因素。阴离子交换色谱法是下游处理和分析不同生物分子时使用的最重要方法之一。本文描述了一种使用对流相互作用介质整体柱来改进残留细胞DNA检测的应用。
