Jones B M, Bird M, Griffin P J, Massey P R, Millar D A, Miller J J, Reeves S, Salaman J R
J Immunol. 1979 Jul;123(1):143-5.
Urine dialysate from rats treated orally with 25 mg/Kg 3H-labeled niridazole was fractionated by DEAE-Sepharose column chromatography and was found to contain three radioactive metabolites and no parent compound. When human niridazole urine dialysate (NUD) was fractionated under identical conditions, fractions corresponding to the three rat NUD metabolites were found to inhibit the human one-way MLR. No inhibition was obtained with fractionated control urine dialysate. It was concluded that nonimmunosuppressive niridazole is metabolized by rats and man to produce three active compounds with the ability to suppress the in vitro response to alloantigens.
用25毫克/千克的3H标记硝唑咪经口处理的大鼠尿液透析液，通过二乙氨基乙基琼脂糖凝胶柱色谱法进行分离，发现含有三种放射性代谢物，且无母体化合物。当在相同条件下对人硝唑咪尿液透析液（NUD）进行分离时，发现与三种大鼠NUD代谢物相对应的组分可抑制人单向混合淋巴细胞反应。分离的对照尿液透析液未产生抑制作用。得出的结论是，非免疫抑制性的硝唑咪在大鼠和人体内代谢，产生三种具有抑制体外对同种异体抗原反应能力的活性化合物。
