甲基化特异性PCR解析
Methylation-specific PCR unraveled.
作者信息
Derks Sarah, Lentjes Marjolein H F M, Hellebrekers Debby M E I, de Bruïne Adriaan P, Herman James G, van Engeland Manon
机构信息
Research Institute GROW, Department of Pathology, University Maastricht, PO Box 616, 6200 MD Maastricht, The Netherlands.
出版信息
Cell Oncol. 2004;26(5-6):291-9. doi: 10.1155/2004/370301.
Methylation-specific PCR (MSP) is a simple, quick and cost-effective method to analyze the DNA methylation status of virtually any group of CpG sites within a CpG island. The technique comprises two parts: (1) sodium bisulfite conversion of unmethylated cytosine's to uracil under conditions whereby methylated cytosines remains unchanged and (2) detection of the bisulfite induced sequence differences by PCR using specific primer sets for both unmethylated and methylated DNA. This review discusses the critical parameters of MSP and presents an overview of the available MSP variants and the (clinical) applications.
甲基化特异性PCR（MSP）是一种简单、快速且经济高效的方法，用于分析CpG岛内几乎任何一组CpG位点的DNA甲基化状态。该技术包括两个部分：（1）在甲基化胞嘧啶保持不变的条件下，将未甲基化的胞嘧啶亚硫酸氢盐转化为尿嘧啶；（2）使用针对未甲基化和甲基化DNA的特异性引物对，通过PCR检测亚硫酸氢盐诱导的序列差异。本文综述讨论了MSP的关键参数，并概述了现有的MSP变体及其（临床）应用。
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