Ong T, Slade B, de Serres F J
J Environ Pathol Toxicol. 1979 Mar-Apr;2(4):1109-18.
Mutagenicity and mutagenic specificity of niridazole and metronidazole, two chemotherapeutic agents used in the treatment of human parasitic diseases, were studied with the ad-3 test system of Neurospora crassa. The results show that neither compound is mutagenic in resting conidia. In growing vegetative cells, however, both compounds are mutagenic in Neurospora crassa. Genetic analysis of the mutants indicate that niridazole induces predominantly base-pair substitution mutations. None of the niridazole-induced mutants resulted from multilocus deletions. The spectra of genetic alterations induced by metronidazole are similar to those induced by the monofunctional alkylating agents, EI, EMS and ICR-177. It is suggested, therefore, that the mechanism of mutation induction by metronidazole in Neurospora is similar to that of monofunctional alkylating agents.
使用粗糙脉孢菌的ad - 3测试系统，研究了用于治疗人类寄生虫病的两种化疗药物硝唑咪和甲硝唑的诱变性及诱变特异性。结果表明，这两种化合物在静止分生孢子中均无诱变性。然而，在生长的营养细胞中，这两种化合物在粗糙脉孢菌中均具有诱变性。对突变体的遗传分析表明，硝唑咪主要诱导碱基对替换突变。没有一个硝唑咪诱导的突变体是由多位点缺失导致的。甲硝唑诱导的遗传改变谱与单功能烷化剂EI、EMS和ICR - 177诱导的相似。因此，有人认为甲硝唑在粗糙脉孢菌中诱导突变的机制与单功能烷化剂相似。
