Neuwelt E A, Gahl W A, Levy C C
Baltimore Cancer Research Center, NCI Laboratory of Molecular Biology, 3100 Wyman Park Drive, Baltimore, Maryland 21211, USA.
Biochem Biophys Res Commun. 1976 Dec 20;73(4):885-93. doi: 10.1016/0006-291x(76)90205-9.
The effect of polyadenylic acid, a potent inhibitor of mammalian and bacterial RNAses, on the binding of human liver RNAse to its antibody was studied. To do this, a human liver RNAse antibody was immobilized on Sepharose 4B. Examination of the ability of the enzyme to bind to the immobilized anti-RNAse in the presence or absence of polyadenylic acid indicated that enzyme-antibody binding was more sensitive to the presence of polyadenylic acid than was enzyme activity. Furthermore, the effect of polyadenylic acid on enzyme-antibody binding was specific since neither polycytidylic acid nor polyuridylic acid had much effect on the antigenicity of the enzyme. The metal cation, Mg2+, and the polyamine, spermidine, but not putrescine, readily reversed the effects of polyadenylic acid on enzyme-antibody binding.
研究了聚腺苷酸（一种对哺乳动物和细菌核糖核酸酶有效的抑制剂）对人肝核糖核酸酶与其抗体结合的影响。为此，将人肝核糖核酸酶抗体固定在琼脂糖4B上。在有或没有聚腺苷酸存在的情况下，检测该酶与固定化抗核糖核酸酶结合的能力，结果表明酶 - 抗体结合对聚腺苷酸的存在比酶活性更敏感。此外，聚腺苷酸对酶 - 抗体结合的影响是特异性的，因为聚胞苷酸和聚尿苷酸对该酶的抗原性都没有太大影响。金属阳离子Mg2 +和多胺亚精胺（而不是腐胺）能轻易逆转聚腺苷酸对酶 - 抗体结合的影响。
