Kurihara M, Ogawa M, Ohta T, Kurokawa E, Kitahara T, Kosaki G, Watanabe T, Wada H
Cancer Res. 1982 Nov;42(11):4836-41.
Three alkaline RNases were purified from human pancreatic juice to near homogeneity as judged by sodium dodecyl sulfate and native polyacrylamide gel electrophoreses. The molecular weights of these RNases determined by sodium dodecyl sulfate electrophoresis were 27,000, 19,000, and 13,000. The activities of these three RNases were increased by addition of Mg2+, Ca2+, Co2+, K+, Na+, spermine, and spermidine and decreased by the addition of heparin, Cu2+, and Zn2+. These RNases showed higher hydrolytic activity toward polycytidylic acid than toward polyguanylic acid, polyuridylic acid, or polyadenylic acid. The three human pancreatic RNases were immunologically identical but differed from human liver RNase, as shown by Ouchterlony double-diffusion test. Radioimmunoassay of human pancreatic RNase showed that immunologically similar RNases are present in human saliva, and serum.
通过十二烷基硫酸钠和天然聚丙烯酰胺凝胶电泳判断，从人胰液中纯化出三种碱性核糖核酸酶，纯度接近均一。通过十二烷基硫酸钠电泳测定，这些核糖核酸酶的分子量分别为27,000、19,000和13,000。添加Mg2+、Ca2+、Co2+、K+、Na+、精胺和亚精胺可增强这三种核糖核酸酶的活性，而添加肝素、Cu2+和Zn2+则会使其活性降低。与聚鸟苷酸、聚尿苷酸或聚腺苷酸相比，这些核糖核酸酶对聚胞苷酸显示出更高的水解活性。如欧氏双扩散试验所示，三种人胰核糖核酸酶在免疫上相同，但与人肝核糖核酸酶不同。人胰核糖核酸酶的放射免疫测定表明，人唾液和血清中存在免疫上相似的核糖核酸酶。
