基因表达微阵列分析可靠吗？视黄酸反应性基因研究综述。
Are gene expression microarray analyses reliable? A review of studies of retinoic acid responsive genes.
作者信息
van der Spek Peter J, Kremer Andreas, Murry Lynn, Walker Michael G
机构信息
Janssen Pharmaceutica, Beerse, Belgium.
出版信息
Genomics Proteomics Bioinformatics. 2003 Feb;1(1):9-14. doi: 10.1016/s1672-0229(03)01003-9.
Abstract
Microarray analyses of gene expression are widely used, but reports of the same analyses by different groups give widely divergent results, and raise questions regarding reproducibility and reliability. We take as an example recent published reports on microarray experiments that were designed to identify retinoic acid responsive genes. These reports show substantial differences in their results. In this article, we review the methodology, results, and potential causes of differences in these applications of microarrays. Finally, we suggest practices to improve the reliability and reproducibility of microarray experiments.
摘要
基因表达的微阵列分析被广泛应用，但不同研究团队对相同分析的报告结果却大相径庭，这引发了关于可重复性和可靠性的质疑。我们以最近发表的关于旨在鉴定视黄酸反应性基因的微阵列实验报告为例。这些报告显示出结果上的显著差异。在本文中，我们回顾了这些微阵列应用中的方法、结果以及差异的潜在原因。最后，我们提出了提高微阵列实验可靠性和可重复性的做法。
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