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基于巨磁阻抗的敏感元件对磁珠的检测。

Magnetic Dynabeads detection by sensitive element based on giant magnetoimpedance.

作者信息

Kurlyandskaya Galina, Levit Vladimir

机构信息

Depto. de Física, Universidad de Oviedo, Avda. Calvo Sotelo s/n, 33007 Oviedo, Spain.

出版信息

Biosens Bioelectron. 2005 Feb 15;20(8):1611-6. doi: 10.1016/j.bios.2004.04.027.


DOI:10.1016/j.bios.2004.04.027
PMID:15626616
Abstract

A GMI-biosensor prototype was designed and fabricated using an amorphous ribbon. As a first step, a GMI response was measured with a model liquid media: suspension containing magnetisable Dynabeads M-450. These beads can be used as biomolecular labels when coated with a specific antibody. The GMI change caused by their presence was measured with a biosensor prototype designed with a measuring cell containing an amorphous CoFeMoSiB-ribbon element. GMI was measured at a range of current frequencies from 0.3 to 10 MHz and at intensities of either Irms=3 or 4.5 mA. Commercial Dynabeads M-450 were supplied as a suspension in a phosphate buffered saline. A maximum difference of 25% in the GMI ratio measured with and without Dynabeads was obtained at a frequency of 3.5 MHz and Irms=3 mA. Some potential applications for GMI-biosensor and further directions are proposed.

摘要

使用非晶带设计并制造了一种巨磁阻抗(GMI)生物传感器原型。第一步,用一种模型液体介质测量GMI响应:含有可磁化的Dynabeads M - 450的悬浮液。当用特定抗体包被时,这些珠子可用作生物分子标记。通过一个带有包含非晶CoFeMoSiB带元件的测量池设计的生物传感器原型来测量由它们的存在引起的GMI变化。在0.3至10 MHz的一系列电流频率以及Irms = 3或4.5 mA的强度下测量GMI。市售的Dynabeads M - 450以磷酸盐缓冲盐水中的悬浮液形式提供。在3.5 MHz频率和Irms = 3 mA时,有和没有Dynabeads测量的GMI比率的最大差异为25%。提出了GMI生物传感器的一些潜在应用和进一步的发展方向。

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