Zeng Xing, Cai Cui, Huang Yu, Ou Ai-Hua, Zhang Xian
Central Laboratory, Guangdong Provincial TCM Hospital, Guangzhou 510120, China.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2005 Jan;21(1):114-7.
To study the function of lymphocytes and the expression pattern of 24 repertoire TCR Vbeta in the convalescent patients with severe acute respiratory syndrome(SARS) after treatment by combination of traditional Chinese and Western medicine.
T lymphocyte subsets, activated states of T and B lymphocytes, and expressions of CD3(+) and 24 TCR Vbeta subfamilies in 76 convalescent SARS patients were detected by flow cytometry.
The percentages of CD3(+) and CD4(+) T cells were lower than normal reference (P<0.05 and P<0.01, respectively). The percentage of CD8(+) T lymphocytes was higher than normal reference (P<0.01),while the percentage of CD8(+)/CD28(-) T cells (Ts) in 49 convalescent SARS patients increased, and that of CD8(+)/CD28(+) T cells (Tc) in 39 patients decreased. The percentage of activated T cells (Ta,CD3(+)/HLA-DR(+)) in 36 convalescent SARS patients and the percentage of activated B cells (Ba, CD3(-)/HLA-DR(+)) in 30 convalescent SARS patients were higher than normal references. Expression of TCR Vbeta14 was the commonest in 24 TCR Vbeta repertoire both in SARS patients and normal controls, followed by TCR Vbeta5.3 in SARS patients, but Vbeta20 in normal controls. Besides, the expressions of TCR Vbeta1, Vbeta5.2, Vbeta5.3, Vbeta7.2, Vbeta9, Vbeta11, Vbeta13.1, Vbeta13.2, Vbeta17, Vbeta18, Vbeta22 and Vbeta23 in SARS group were all significantly higher than those in normal group. And the expressions of TCR Vbeta4, Vbeta22 and Vbeta23 in SARS patients treated with glucocorticoid(>1 000 U) were higher than those in SARS patients without treatment.
Increased Ts cell may lead to increased CD8(+) T cells in convalescent SARS patients. The suppressor factors secreted by Ts cells may decrease CD3(+) and CD4(+) T cells. Expression of TCR Vbeta on T cells from convalescent SARS patients were different from normal controls. The increased Ta and Ba expressions may be associated with activation of T and B cells.
研究中西医结合治疗后重症急性呼吸综合征（SARS）康复患者淋巴细胞功能及24种TCR Vβ谱型的表达模式。
采用流式细胞术检测76例SARS康复患者的T淋巴细胞亚群、T和B淋巴细胞活化状态以及CD3(+)和24种TCR Vβ亚家族的表达。
CD3(+)和CD4(+) T细胞百分比低于正常参考值（分别为P<0.05和P<0.01）。CD8(+) T淋巴细胞百分比高于正常参考值（P<0.01），49例SARS康复患者中CD8(+)/CD28(-) T细胞（Ts）百分比升高，39例患者中CD8(+)/CD28(+) T细胞（Tc）百分比降低。36例SARS康复患者中活化T细胞（Ta，CD3(+)/HLA-DR(+)）百分比和30例SARS康复患者中活化B细胞（Ba，CD3(-)/HLA-DR(+)）百分比高于正常参考值。TCR Vβ14在SARS患者和正常对照的24种TCR Vβ谱型中表达最为常见，SARS患者中其次为TCR Vβ5.3，正常对照中为Vβ20。此外，SARS组中TCR Vβ1、Vβ5.2、Vβ5.3、Vβ7.2、Vβ9、Vβ11、Vβ13.1、Vβ13.2、Vβ17、Vβ18、Vβ22和Vβ23的表达均显著高于正常组。使用糖皮质激素（>1 000 U）治疗的SARS患者中TCR Vβ4、Vβ22和Vβ23的表达高于未治疗的SARS患者。
SARS康复患者中Ts细胞增加可能导致CD8(+) T细胞增多。Ts细胞分泌的抑制因子可能使CD3(+)和CD4(+) T细胞减少。SARS康复患者T细胞上TCR Vβ的表达与正常对照不同。Ta和Ba表达增加可能与T和B细胞活化有关。
