沙眼衣原体中依赖N5-谷氨酰胺S-腺苷-L-甲硫氨酸的甲基转移酶PrmC/HemK可使1类释放因子发生甲基化。
The N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase PrmC/HemK in Chlamydia trachomatis methylates class 1 release factors.
作者信息
Pannekoek Yvonne, Heurgué-Hamard Valérie, Langerak Ankie A J, Speijer Dave, Buckingham Richard H, van der Ende Arie
机构信息
Academic Medical Center, Dept. of Medical Microbiology, Room L1-162, University of Amsterdam, PO Box 22660, 1100 DD Amsterdam, The Netherlands.
出版信息
J Bacteriol. 2005 Jan;187(2):507-11. doi: 10.1128/JB.187.2.507-511.2005.
Abstract
The gene prmC, encoding the putative S-adenosyl-L-methionine (AdoMet)-dependent methyltransferase (MTase) of release factors (RFs) of the obligate intracellular pathogen Chlamydia trachomatis, was functionally analyzed. Chlamydial PrmC expression suppresses the growth defect of a prmC knockout strain of Escherichia coli K-12, suggesting an interaction of chlamydial PrmC with E. coli RFs in vivo. In vivo methylation assays carried out with recombinant PrmC and RFs of chlamydial origin demonstrated that PrmC methylates RFs within the tryptic fragment containing the universally conserved sequence motif Gly-Gly-Gln. This is consistent with the enzymatic properties of PrmC of E. coli origin. We conclude that C. trachomatis PrmC functions as an N5-glutamine AdoMet-dependent MTase, involved in methylation of RFs.
摘要
对编码专性胞内病原体沙眼衣原体释放因子（RFs）假定的依赖S-腺苷-L-甲硫氨酸（AdoMet）的甲基转移酶（MTase）的基因prmC进行了功能分析。衣原体PrmC的表达抑制了大肠杆菌K-12的prmC基因敲除菌株的生长缺陷，这表明衣原体PrmC在体内与大肠杆菌RFs存在相互作用。用重组PrmC和衣原体来源的RFs进行的体内甲基化试验表明，PrmC使包含普遍保守序列基序甘氨酸-甘氨酸-谷氨酰胺的胰蛋白酶片段内的RFs发生甲基化。这与大肠杆菌来源的PrmC的酶学特性一致。我们得出结论，沙眼衣原体PrmC作为一种依赖N5-谷氨酰胺AdoMet的MTase发挥作用，参与RFs的甲基化。
相似文献
1
The N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase PrmC/HemK in Chlamydia trachomatis methylates class 1 release factors.沙眼衣原体中依赖N5-谷氨酰胺S-腺苷-L-甲硫氨酸的甲基转移酶PrmC/HemK可使1类释放因子发生甲基化。
J Bacteriol. 2005 Jan;187(2):507-11. doi: 10.1128/JB.187.2.507-511.2005.
2
HemK, a class of protein methyl transferase with similarity to DNA methyl transferases, methylates polypeptide chain release factors, and hemK knockout induces defects in translational termination.HemK是一类与DNA甲基转移酶相似的蛋白质甲基转移酶，它能使多肽链释放因子发生甲基化，并且HemK基因敲除会导致翻译终止缺陷。
Proc Natl Acad Sci U S A. 2002 Feb 5;99(3):1473-8. doi: 10.1073/pnas.032488499. Epub 2002 Jan 22.
3
Molecular basis for bacterial class I release factor methylation by PrmC.PrmC介导细菌I类释放因子甲基化的分子基础。
Mol Cell. 2005 Dec 22;20(6):917-27. doi: 10.1016/j.molcel.2005.10.025.
4
Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferase.PrmC/HemK催化途径中的结构，一种N5-谷氨酰胺腺苷甲硫氨酸依赖性甲基转移酶。
Biochemistry. 2003 May 20;42(19):5592-9. doi: 10.1021/bi034026p.
5
HemK2 protein, encoded on human chromosome 21, methylates translation termination factor eRF1.人类21号染色体上编码的HemK2蛋白可使翻译终止因子eRF1发生甲基化。
FEBS Lett. 2008 Jul 9;582(16):2352-6. doi: 10.1016/j.febslet.2008.05.045. Epub 2008 Jun 6.
6
The hemK gene in Escherichia coli encodes the N(5)-glutamine methyltransferase that modifies peptide release factors.大肠杆菌中的hemK基因编码修饰肽释放因子的N(5)-谷氨酰胺甲基转移酶。
EMBO J. 2002 Feb 15;21(4):769-78. doi: 10.1093/emboj/21.4.769.
7
The chlamydial functional homolog of KsgA confers kasugamycin sensitivity to Chlamydia trachomatis and impacts bacterial fitness.沙眼衣原体 KsgA 的功能同源物赋予了杀霉素敏感性，并影响了细菌的适应性。
BMC Microbiol. 2009 Dec 31;9:279. doi: 10.1186/1471-2180-9-279.
8
Is the HemK family of putative S-adenosylmethionine-dependent methyltransferases a "missing" zeta subfamily of adenine methyltransferases? A hypothesis.假定的依赖S-腺苷甲硫氨酸的甲基转移酶HemK家族是腺嘌呤甲基转移酶的一个“缺失”的ζ亚家族吗？一种假说。
IUBMB Life. 1999 Sep;48(3):247-9. doi: 10.1080/713803519.
9
Catalytic properties and kinetic mechanism of human recombinant Lys-9 histone H3 methyltransferase SUV39H1: participation of the chromodomain in enzymatic catalysis.人重组赖氨酸-9组蛋白H3甲基转移酶SUV39H1的催化特性及动力学机制：色域在酶催化中的作用
Biochemistry. 2006 Mar 14;45(10):3272-84. doi: 10.1021/bi051997r.
10
Structural characterization and comparative phylogenetic analysis of Escherichia coli HemK, a protein (N5)-glutamine methyltransferase.蛋白质（N5）-谷氨酰胺甲基转移酶大肠杆菌HemK的结构表征及比较系统发育分析
J Mol Biol. 2004 Jul 16;340(4):695-706. doi: 10.1016/j.jmb.2004.05.019.
引用本文的文献
1
Cross-Reactivity of N6AMT1 Antibodies with Aurora Kinase A: An Example of Antibody-Specific Non-Specificity.N6AMT1抗体与极光激酶A的交叉反应性：抗体特异性非特异性的一个例子
Antibodies (Basel). 2024 Apr 22;13(2):33. doi: 10.3390/antib13020033.
2
The methyltransferase N6AMT1 participates in the cell cycle by regulating cyclin E levels.甲基转移酶 N6AMT1 通过调节细胞周期蛋白 E 水平参与细胞周期。
PLoS One. 2024 Feb 23;19(2):e0298884. doi: 10.1371/journal.pone.0298884. eCollection 2024.
3
Expanding the Structural Diversity of Drimentines by Exploring the Promiscuity of Two N-methyltransferases.通过探索两种N-甲基转移酶的底物选择性来扩展苦地胆素的结构多样性
iScience. 2020 Jul 24;23(7):101323. doi: 10.1016/j.isci.2020.101323. Epub 2020 Jun 28.
4
The feather degradation mechanisms of a new Streptomyces sp. isolate SCUT-3.一株新型链霉菌 SCUT-3 的羽毛降解机制。
Commun Biol. 2020 Apr 24;3(1):191. doi: 10.1038/s42003-020-0918-0.
5
The endosymbiont Amoebophilus asiaticus encodes an S-adenosylmethionine carrier that compensates for its missing methylation cycle.亚洲内共生阿米巴体编码一个 S-腺苷甲硫氨酸载体，可补偿其缺失的甲基化循环。
J Bacteriol. 2013 Jul;195(14):3183-92. doi: 10.1128/JB.00195-13. Epub 2013 May 10.
6
Identification and characterization of the Chlamydia trachomatis L2 S-adenosylmethionine transporter.鉴定和表征沙眼衣原体 L2 的 S-腺苷甲硫氨酸转运蛋白。
mBio. 2011 May 10;2(3):e00051-11. doi: 10.1128/mBio.00051-11. Print 2011.
7
The chlamydial functional homolog of KsgA confers kasugamycin sensitivity to Chlamydia trachomatis and impacts bacterial fitness.沙眼衣原体 KsgA 的功能同源物赋予了杀霉素敏感性，并影响了细菌的适应性。
BMC Microbiol. 2009 Dec 31;9:279. doi: 10.1186/1471-2180-9-279.
8
Molecular characterization and identification of proteins regulated by Hfq in Neisseria meningitidis.脑膜炎奈瑟菌中受Hfq调控的蛋白质的分子特征及鉴定
FEMS Microbiol Lett. 2009 May;294(2):216-24. doi: 10.1111/j.1574-6968.2009.01568.x. Epub 2009 Apr 3.
9
Cloning and primarily function study of two novel putative N5-glutamine methyltransferase (Hemk) splice variants from mouse stem cells.从鼠干细胞中克隆和初步研究两种新型假定的 N5-谷氨酰胺甲基转移酶（Hemk）剪接变异体。
Mol Biol Rep. 2009 Nov;36(8):2221-8. doi: 10.1007/s11033-008-9437-7. Epub 2008 Dec 30.
10
Chloroplast ribosome release factor 1 (AtcpRF1) is essential for chloroplast development.叶绿体核糖体释放因子1（AtcpRF1）对叶绿体发育至关重要。
Plant Mol Biol. 2007 Jul;64(5):481-97. doi: 10.1007/s11103-007-9166-7. Epub 2007 Apr 21.
本文引用的文献
1
Many paths to methyltransfer: a chronicle of convergence.通往甲基转移的多条途径：融合纪事
Trends Biochem Sci. 2003 Jun;28(6):329-35. doi: 10.1016/S0968-0004(03)00090-2.
2
Genomic transcriptional profiling of the developmental cycle of Chlamydia trachomatis.沙眼衣原体发育周期的基因组转录谱分析。
Proc Natl Acad Sci U S A. 2003 Jul 8;100(14):8478-83. doi: 10.1073/pnas.1331135100. Epub 2003 Jun 18.
3
Structures along the catalytic pathway of PrmC/HemK, an N5-glutamine AdoMet-dependent methyltransferase.PrmC/HemK催化途径中的结构，一种N5-谷氨酰胺腺苷甲硫氨酸依赖性甲基转移酶。
Biochemistry. 2003 May 20;42(19):5592-9. doi: 10.1021/bi034026p.
4
An epigenetic road map for histone lysine methylation.组蛋白赖氨酸甲基化的表观遗传路线图。
J Cell Sci. 2003 Jun 1;116(Pt 11):2117-24. doi: 10.1242/jcs.00493.
5
Global stage-specific gene regulation during the developmental cycle of Chlamydia trachomatis.沙眼衣原体发育周期中的全局阶段特异性基因调控。
J Bacteriol. 2003 May;185(10):3179-89. doi: 10.1128/JB.185.10.3179-3189.2003.
6
S-adenosylmethionine transport in Rickettsia prowazekii.普氏立克次体中的S-腺苷甲硫氨酸转运
J Bacteriol. 2003 May;185(10):3031-5. doi: 10.1128/JB.185.10.3031-3035.2003.
7
Genome sequence of Chlamydophila caviae (Chlamydia psittaci GPIC): examining the role of niche-specific genes in the evolution of the Chlamydiaceae.豚鼠嗜衣原体（鹦鹉热衣原体GPIC）的基因组序列：研究生态位特异性基因在衣原体科进化中的作用。
Nucleic Acids Res. 2003 Apr 15;31(8):2134-47. doi: 10.1093/nar/gkg321.
8
Termination of translation: interplay of mRNA, rRNAs and release factors?翻译的终止：信使核糖核酸、核糖体核糖核酸与释放因子的相互作用？
EMBO J. 2003 Jan 15;22(2):175-82. doi: 10.1093/emboj/cdg017.
9
Receptor methylation controls the magnitude of stimulus-response coupling in bacterial chemotaxis.受体甲基化控制细菌趋化作用中刺激-反应偶联的强度。
J Biol Chem. 2002 Sep 27;277(39):36760-5. doi: 10.1074/jbc.M204325200. Epub 2002 Jul 15.
10
Beyond Watson and Crick: DNA methylation and molecular enzymology of DNA methyltransferases.超越沃森和克里克：DNA甲基化与DNA甲基转移酶的分子酶学
Chembiochem. 2002 Apr 2;3(4):274-93. doi: 10.1002/1439-7633(20020402)3:4<274::AID-CBIC274>3.0.CO;2-S.
Zotero 插件