School of Biology and Biological Engineering, South China University of Technology, Guangzhou, Guangdong, P. R. China.
Yingdong College of Life Sciences, Shaoguan University, Shaoguan, Guangdong, P. R. China.
Commun Biol. 2020 Apr 24;3(1):191. doi: 10.1038/s42003-020-0918-0.
Feather waste is the highest protein-containing resource in nature and is poorly reused. Bioconversion is widely accepted as a low-cost and environmentally benign process, but limited by the availability of safe and highly efficient feather degrading bacteria (FDB) for its industrial-scale fermentation. Excessive focuses on keratinase and limited knowledge of other factors have hindered complete understanding of the mechanisms employed by FDB to utilize feathers and feather cycling in the biosphere. Streptomyces sp. SCUT-3 can efficiently degrade feather to products with high amino acid content, useful as a nutrition source for animals, plants and microorganisms. Using multiple omics and other techniques, we reveal how SCUT-3 turns on its feather utilization machinery, including its colonization, reducing agent and protease secretion, peptide/amino acid importation and metabolism, oxygen consumption and iron uptake, spore formation and resuscitation, and so on. This study would shed light on the feather utilization mechanisms of FDBs.
羽毛废料是自然界中蛋白质含量最高的资源，但利用率很低。生物转化被广泛认为是一种低成本且环境友好的工艺，但由于缺乏安全且高效的羽毛降解菌（FDB），其工业规模发酵受到限制。过度关注角蛋白酶和对其他因素的了解有限，阻碍了对 FDB 利用羽毛和羽毛在生物圈中循环的机制的全面理解。链霉菌 SCUT-3 可以有效地将羽毛降解为具有高氨基酸含量的产物，可用作动物、植物和微生物的营养源。使用多种组学和其他技术，我们揭示了 SCUT-3 如何启动其羽毛利用机制，包括其定殖、还原剂和蛋白酶的分泌、肽/氨基酸的摄取和代谢、耗氧量和铁摄取、孢子形成和复苏等。这项研究将阐明 FDB 利用羽毛的机制。
