Abu-Zeid Y A, Theander T G, Abdulhadi N H, Hviid L, Saeed B O, Jepsen S, Jensen J B, Bayoumi R A
Department of Biochemistry, Faculty of Medicine, University of Khartoum, Sudan.
Clin Exp Immunol. 1992 Apr;88(1):112-8. doi: 10.1111/j.1365-2249.1992.tb03048.x.
Plasma and peripheral blood mononuclear cells (PBMC) were obtained from P. falciparum-infected individuals with and without the sickle cell trait at diagnosis and 7 days after treatment. HbAA and HbAS patients were compared for levels of plasma soluble IL-2 receptors (IL-2R) and the in vitro cellular reactivity to affinity-purified soluble P. falciparum antigens (SPAg), PPD and phytohaemagglutinin (PHA). At diagnosis, HbAS patients with clinical disease had lower plasma-soluble IL-2R levels and parasite counts than the corresponding HbAA patients, whereas HbAS and HbAA patients with asymptomatic infections had comparable soluble IL-2R levels and parasite counts. PBMC from HbAS patients had higher proliferation and IFN-gamma production in response to SPAg than PBMC from HbAA patients. The difference in the lymphoproliferative responses to SPAg between the two groups was evident in patients with asymptomatic infections. In all patients, the clinical severity, the soluble IL-2R levels and the parasite counts were directly related. The former two were inversely related to the proliferative responses to SPAg. After treatment, all the studied parameters were comparable in the two groups. The study indicates that during P. falciparum infection, HbAS compared with HbAA patients had lower in vivo cellular activation and higher in vitro cellular reactivity in response to soluble malaria antigens.
在诊断时以及治疗后7天，从患有和未患有镰状细胞性状的恶性疟原虫感染个体中获取血浆和外周血单核细胞（PBMC）。比较了HbAA和HbAS患者的血浆可溶性白细胞介素-2受体（IL-2R）水平以及对亲和纯化的可溶性恶性疟原虫抗原（SPAg）、结核菌素纯蛋白衍生物（PPD）和植物血凝素（PHA）的体外细胞反应性。在诊断时，患有临床疾病的HbAS患者的血浆可溶性IL-2R水平和寄生虫计数低于相应的HbAA患者，而无症状感染的HbAS和HbAA患者的可溶性IL-2R水平和寄生虫计数相当。与HbAA患者的PBMC相比，HbAS患者的PBMC对SPAg的增殖和干扰素-γ产生更高。两组对SPAg的淋巴细胞增殖反应差异在无症状感染患者中很明显。在所有患者中，临床严重程度、可溶性IL-2R水平和寄生虫计数直接相关。前两者与对SPAg的增殖反应呈负相关。治疗后，两组中所有研究参数相当。该研究表明，在恶性疟原虫感染期间，与HbAA患者相比，HbAS患者体内细胞活化较低，而对可溶性疟疾抗原的体外细胞反应性较高。
