Margolis R U, Lalley K, Kiang W L, Crockett C, Margolis R K
Department of Pharmacology, New York University School of Medicine, New York, NY 10016, USA.
Biochem Biophys Res Commun. 1976 Dec 20;73(4):1018-24. doi: 10.1016/0006-291x(76)90224-2.
A proteoglycan in which the glycosaminoglycans are predominantly chondroitin sulfate has been isolated from the soluble fraction of rat brain by ion exchange chromatography and gel filtration. Glycoprotein oligosaccharides are also present, and result in adsorption of the proteoglycan by Concanavalin A-Sepharose. The proteoglycan-glycoprotein complex eluted from the affinity column by alpha-methylglucoside floats near the top of a cesium chloride density gradient run under dissociative conditions (4 M guanidine), but after beta-elimination of the chondroitin sulfate polysaccharide chains from their low buoyant density glycoprotein complex they sediment to the bottom of the gradient. These results suggest that relatively few polysaccharide chains are covalently linked to a large protein core in the dissociated chondroitin sulfate proteoglycan "subunit" from brain, and that the proteoglycans are closely associated with soluble glycoproteins.
通过离子交换色谱法和凝胶过滤法，从大鼠脑的可溶部分分离出一种蛋白聚糖，其中糖胺聚糖主要为硫酸软骨素。同时也存在糖蛋白寡糖，这导致该蛋白聚糖被伴刀豆球蛋白A - 琼脂糖吸附。从亲和柱上用α - 甲基葡萄糖苷洗脱下来的蛋白聚糖 - 糖蛋白复合物，在解离条件（4M胍）下进行的氯化铯密度梯度离心中漂浮在接近顶部的位置，但在从其低浮力密度的糖蛋白复合物中β - 消除硫酸软骨素多糖链后，它们沉淀到梯度底部。这些结果表明，在从脑中解离出的硫酸软骨素蛋白聚糖“亚基”中，相对较少的多糖链与一个大的蛋白质核心共价连接，并且这些蛋白聚糖与可溶性糖蛋白紧密相关。
