以硫酸软骨素蛋白聚糖形式存在的多种受体样蛋白酪氨酸磷酸酶。
Multiple receptor-like protein tyrosine phosphatases in the form of chondroitin sulfate proteoglycan.
作者信息
Maeda N, Hamanaka H, Shintani T, Nishiwaki T, Noda M
机构信息
Division of Molecular Neurobiology, National Institute for Basic Biology, Okazaki, Japan.
出版信息
FEBS Lett. 1994 Oct 31;354(1):67-70. doi: 10.1016/0014-5793(94)01093-5.
The possibility that some of the brain proteoglycans are receptor-like protein tyrosine phosphatases (PTPases) was investigated. Membrane-bound proteoglycan fractions were prepared from the postnuclear membrane fraction of 8-day-old rat brain by DEAE ion-exchange chromatography and CsCl density gradient centrifugation. The isolated proteoglycan fractions showed high PTPase specific activities together with the typical PTPase characteristics. Renaturation experiments indicated that chondroitin sulfate proteoglycans with 380- and 170-kDa core proteins carried the PTPase activity. The proteoglycan with 380-kDa core protein was identified as RPTP beta/zeta bearing HNK-1 carbohydrate.
研究了某些脑蛋白聚糖是否为受体样蛋白酪氨酸磷酸酶（PTPases）的可能性。通过DEAE离子交换色谱法和CsCl密度梯度离心法，从8日龄大鼠脑的核后膜部分制备了膜结合蛋白聚糖组分。分离得到的蛋白聚糖组分表现出高PTPase比活性以及典型的PTPase特征。复性实验表明，具有380 kDa和170 kDa核心蛋白的硫酸软骨素蛋白聚糖具有PTPase活性。具有380 kDa核心蛋白的蛋白聚糖被鉴定为带有HNK-1碳水化合物的RPTPβ/ζ。
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