Jiang Su, Chen Cai-Yan, Cheng Zhu-Kuan, Cai Run, Zhai Wen-Xue, Zhu Li-Huang
Department of Botanical Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 201101, China.
Yi Chuan Xue Bao. 2004 Dec;31(12):1381-7.
Rice calli derived from anther culture were used as recipient to transfer a rice blight resistance gene, Xa21, into a japonica rice variety, Taipei 309, via Agrobacterium-mediated transformation. Seven green transgenic plants, including one mixoploid, two haploid, and four diploid plants, were regenerated. PCR, Southern blot, FISH and blight resistance analysis all indicated that Xa21 gene has been integrated into the T0 plant genomes. T1 generations of the four diploid T0 plants were further investigated for resistance segregation. Chi2 test showed that two T1 populations segregated with a ratio of 3:1, indicating that a single copy of Xa21 gene was integrated into the genome, whereas the segregation ratios of the other two T1 populations were non-Mendelian. Therefore, the four diploid transgenic plants should be heterozygous diploids.
以花药培养获得的水稻愈伤组织为受体，通过农杆菌介导的转化，将水稻白叶枯病抗性基因Xa21导入粳稻品种台北309中。再生出了7株绿色转基因植株，包括1株混倍体、2株单倍体和4株二倍体植株。PCR、Southern杂交、荧光原位杂交（FISH）和白叶枯病抗性分析均表明，Xa21基因已整合到T0代植株基因组中。对4株二倍体T0代植株的T1代进一步进行抗性分离研究。卡方检验表明，两个T1群体的分离比例为3:1，表明Xa21基因单拷贝整合到基因组中，而另外两个T1群体的分离比例不符合孟德尔遗传规律。因此，这4株二倍体转基因植株应为杂合二倍体。
