Wu Guan-Ting, Lang Chun-Xiu, Hu Zhang-Hua, Chen Xiao-Yun, Wang Fu-Lin, Jin Wei, Chen Jin-Qing
Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao. 2006 Jun;32(3):339-44.
Gene for phosphoenolpyruvate carboxylase (PEPCase) was incorporated in antisense orientation into genome of japonica rice variety Xiushui 11 through Agrobacterium tumefaciens-mediated transformation of mature seeds-derived embryogenic calli, and a total of 95 transgenic plants were regenerated, which were confirmed by histochemical detection of GUS activity (Fig.2), PCR assay (Fig.3) and Southern hybridization analysis (Fig.4). The transgene was inherited by the T1 progeny and Mendelian 3:1 segregation ratio was observed in most transgenic lines. It was found that the fat content of hulled grains of T2 transgenic lines was 0.37 +/- 0.12 per cent higher than that of the control, most of which were statistically very significant (Table 2).
通过根癌农杆菌介导的成熟种子来源的胚性愈伤组织转化，将磷酸烯醇式丙酮酸羧化酶（PEPCase）基因以反义方向整合到粳稻品种秀水11的基因组中，共再生出95株转基因植株，通过GUS活性的组织化学检测（图2）、PCR分析（图3）和Southern杂交分析（图4）对其进行了确认。转基因由T1代子代遗传，在大多数转基因株系中观察到孟德尔3:1的分离比。发现T2转基因株系糙米的脂肪含量比对照高0.37±0.12%，其中大部分在统计学上差异极显著（表2）。
