采用E试验和棋盘法对针对羊布鲁氏菌的抗菌药物组合进行协同试验。
Synergy tests by E test and checkerboard methods of antimicrobial combinations against Brucella melitensis.
作者信息
Orhan Gani, Bayram Aysen, Zer Yasemin, Balci Iclal
机构信息
Gaziantep University Medical Faculty, Department of Microbiology and Clinical Microbiology, Universite Bulvari, 27310 Gaziantep, Turkey.
出版信息
J Clin Microbiol. 2005 Jan;43(1):140-3. doi: 10.1128/JCM.43.1.140-143.2005.
Abstract
Two different synergy testing methods, the checkerboard and the E test methods, were used to compare the in vitro efficacies of various antimicrobial combinations against 16 Brucella melitensis strains isolated from blood cultures. The rate of agreement of the E test and checkerboard methods was found to be 55%. The most concordant results were found for the streptomycin-doxycycline combination in 12 (75%) tests, in which four strains showed synergistic activity by E test and antagonistic activity by the checkerboard method and in which one strain showed antagonistic activity by both methods. Even though each of these methods uses different conditions and endpoints, the results of both methods frequently agreed.
摘要
采用两种不同的协同试验方法，棋盘法和E试验法，比较了多种抗菌药物组合对从血培养中分离出的16株羊布鲁氏菌的体外抗菌效果。发现E试验法和棋盘法的一致率为55%。在12次（75%）试验中，链霉素-强力霉素组合的结果最为一致，其中4株菌通过E试验显示协同活性，通过棋盘法显示拮抗活性，1株菌在两种方法中均显示拮抗活性。尽管这两种方法采用了不同的条件和终点，但两种方法的结果经常一致。
相似文献
1
Synergy tests by E test and checkerboard methods of antimicrobial combinations against Brucella melitensis.采用E试验和棋盘法对针对羊布鲁氏菌的抗菌药物组合进行协同试验。
J Clin Microbiol. 2005 Jan;43(1):140-3. doi: 10.1128/JCM.43.1.140-143.2005.
2
Tigecycline: its potential for treatment of brucellosis.替加环素：其治疗布鲁氏菌病的潜力。
Scand J Infect Dis. 2007;39(5):432-4. doi: 10.1080/00365540601105756.
3
Biotypes and antimicrobial susceptibilities of Brucella isolates.布鲁氏菌分离株的生物型及抗菌药敏性
Scand J Infect Dis. 2003;35(5):337-8. doi: 10.1080/00365540310008348.
4
Determining in vitro synergistic activities of tigecycline with several other antibiotics against Brucella melitensis using checkerboard and time-kill assays.采用棋盘法和时间杀菌试验测定替加环素与其他几种抗生素对羊种布鲁氏菌的体外协同活性。
J Chemother. 2009 Feb;21(1):24-30. doi: 10.1179/joc.2009.21.1.24.
5
In vitro efficacy of several antimicrobial combinations against Brucella canis and Brucella melitensis strains isolated from dogs.几种抗菌药物组合对从犬分离出的犬布鲁氏菌和羊布鲁氏菌菌株的体外疗效。
Vet Microbiol. 1995 Jun;45(1):1-10. doi: 10.1016/0378-1135(94)00122-d.
6
The effect of tigecycline and ertapenem against clinical isolates of Brucella melitensis detected by E-test on different media.采用E试验检测替加环素和厄他培南对不同培养基上布鲁氏菌临床分离株的作用。
Indian J Med Microbiol. 2010 Oct-Dec;28(4):370-1. doi: 10.4103/0255-0857.71822.
7
8
9
Efficacy of several antibiotic combinations against Brucella melitensis Rev 1 experimental infection in BALB/c mice.几种抗生素组合对BALB/c小鼠布鲁氏菌Melitensis Rev 1实验性感染的疗效。
J Antimicrob Chemother. 2006 Sep;58(3):622-6. doi: 10.1093/jac/dkl289. Epub 2006 Jul 18.
10
[Comparison of the checkerboard and E-test methods used for the analysis of two antibiotics combination].[用于分析两种抗生素联合使用的棋盘法和E试验法的比较]
Med Dosw Mikrobiol. 2005;57(1):65-75.
引用本文的文献
1
An Evaluation of Alternative Treatment Strategies in Mitigating Colistin Resistance: Targeting Plasmid Transfer Through the Use of Bambermycin or the Protein Coded by the Mcr-1 Gene With Antibodies and Streptomycin.减轻黏菌素耐药性的替代治疗策略评估：通过使用班贝霉素或用抗体和链霉素靶向Mcr-1基因编码的蛋白质来靶向质粒转移。
Vet Med Sci. 2025 Sep;11(5):e70519. doi: 10.1002/vms3.70519.
2
Synergistic effect of the hybrid compounds of cationic pillararene and an efflux pump inhibitor against Gram-negative bacteria.阳离子柱芳烃与外排泵抑制剂杂化化合物对革兰氏阴性菌的协同作用。
RSC Med Chem. 2025 Jun 4. doi: 10.1039/d5md00262a.
3
Bactericidal activity of mammalian histones is caused by large membrane pore formation.哺乳动物组蛋白的杀菌活性是由大的膜孔形成引起的。
Cell Rep. 2025 May 27;44(5):115658. doi: 10.1016/j.celrep.2025.115658. Epub 2025 May 6.
4
Synergistic Antibacterial Effect of Mucus Fraction from and Cirpofloxacin Against Pathogenic Bacteria Isolated from Wounds of Diabetic Patients.糖尿病患者伤口分离出的病原菌对环丙沙星与黏液成分的协同抗菌作用
Antibiotics (Basel). 2025 Mar 4;14(3):260. doi: 10.3390/antibiotics14030260.
5
Bioactivities of secondary metabolites of two actinomycetes Streptomyces parvulus GloL3, and Streptomyces lienomycini SK5, endophytes of two Indian medicinal herbs- Globba marantina L. and Selaginella kraussiana (Kunze) A. Braun.两种放线菌——微小链霉菌GloL3和链霉菌SK5（两种印度药用植物——姜黄球根状姜和矮泽米苏铁的内生菌）次生代谢产物的生物活性
Braz J Microbiol. 2025 Jun;56(2):779-795. doi: 10.1007/s42770-025-01615-y. Epub 2025 Jan 29.
6
evaluation of tigecycline synergy testing with nine antimicrobial agents against clinical strains.替加环素与九种抗菌药物对临床菌株的协同试验评估
Front Microbiol. 2024 Oct 18;15:1490032. doi: 10.3389/fmicb.2024.1490032. eCollection 2024.
7
Synthesis and antibacterial potential of novel thymol derivatives against methicillin-resistant and pathogenic bacteria.新型百里香酚衍生物对耐甲氧西林和病原菌的合成及其抗菌潜力
Front Chem. 2024 Oct 16;12:1482852. doi: 10.3389/fchem.2024.1482852. eCollection 2024.
8
Imidazoles and Quaternary Ammonium Compounds as Effective Therapies against (Multidrug-Resistant) Bacterial Wound Infections.咪唑类和季铵化合物作为治疗（多重耐药）细菌性伤口感染的有效疗法
Antibiotics (Basel). 2024 Oct 10;13(10):949. doi: 10.3390/antibiotics13100949.
9
Combinatory Use of hLF(1-11), a Synthetic Peptide Derived from Human Lactoferrin, and Fluconazole/Amphotericin B against Reveals a Synergistic/Additive Antifungal Effect.人乳铁蛋白衍生的合成肽hLF(1-11)与氟康唑/两性霉素B联合使用对[具体对象未给出]显示出协同/相加抗真菌作用。
Antibiotics (Basel). 2024 Aug 22;13(8):790. doi: 10.3390/antibiotics13080790.
10
Dihydrothiazolo ring-fused 2-pyridone antimicrobial compounds treat skin and soft tissue infection.二氢噻唑并并吡啶酮类抗菌化合物治疗皮肤和软组织感染。
Sci Adv. 2024 Aug 2;10(31):eadn7979. doi: 10.1126/sciadv.adn7979.
本文引用的文献
1
Evaluation of antibiotic synergy against Acinetobacter baumannii: a comparison with Etest, time-kill, and checkerboard methods.评估抗生素对鲍曼不动杆菌的协同作用：与Etest法、时间杀菌法和棋盘法的比较。
Diagn Microbiol Infect Dis. 2000 Sep;38(1):43-50. doi: 10.1016/s0732-8893(00)00163-2.
2
Activities of levofloxacin, ofloxacin, and ciprofloxacin, alone and in combination with amikacin, against acinetobacters as determined by checkerboard and time-kill studies.通过棋盘法和时间杀菌研究测定左氧氟沙星、氧氟沙星和环丙沙星单独及与阿米卡星联合使用时对不动杆菌的活性。
Antimicrob Agents Chemother. 1997 May;41(5):1073-6. doi: 10.1128/AAC.41.5.1073.
3
Comparison of three different in vitro methods of detecting synergy: time-kill, checkerboard, and E test.三种不同体外检测协同作用方法的比较：时间杀菌法、棋盘法和E试验。
Antimicrob Agents Chemother. 1996 Aug;40(8):1914-8. doi: 10.1128/AAC.40.8.1914.
Zotero 插件