牛膝菊黄酮化合物 Nepetin 的细胞毒性活性
Cytotoxic activity of nepetin, a flavonoid from Eupatorium ballotaefolium HBK.
作者信息
Militão G C G, Albuquerque M R J R, Pessoa O D L, Pessoa C, Moraes M E A, de Moraes M O, Costa-Lotufo L V
机构信息
Curso de Pós-Graduação em Farmacologia, Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceará, Fortaleza, Ceará, Brasil.
出版信息
Pharmazie. 2004 Dec;59(12):965-6.
The present study evaluated the cytotoxic activity of nepetin and quercetin-3-O-glucoside, compounds isolated from the aerial parts of Eupatorium ballotaefolium. The antimitotic activity was determined as the ability to inhibit sea urchin eggs development and five tumor cells lines growth. Moreover, the activities of these compounds were compared to quercetin in the same models. Nepetin inhibited the proliferation of the five tumor cell lines, once quercetin-3-O-glucoside did not present any activity even at the highest tested concentration and quercetin only inhibited proliferation of the B16 cell line. On the sea urchin assay, nepetin and quercetin induced a dose-dependent inhibition on egg development, while quercetin-3-O-glucoside did not modify normalegg cleavage, even at the highest tested concentration (100 microg/ml).
本研究评估了从球花泽兰地上部分分离得到的化合物 Nepetin 和槲皮素 -3-O- 葡萄糖苷的细胞毒性活性。抗有丝分裂活性通过抑制海胆卵发育和五种肿瘤细胞系生长的能力来确定。此外，在相同模型中，将这些化合物的活性与槲皮素进行了比较。Nepetin 抑制了五种肿瘤细胞系的增殖，而槲皮素 -3-O- 葡萄糖苷即使在最高测试浓度下也没有任何活性，槲皮素仅抑制了 B16 细胞系的增殖。在海胆试验中，Nepetin 和槲皮素对卵发育呈现剂量依赖性抑制，而槲皮素 -3-O- 葡萄糖苷即使在最高测试浓度（100 微克 / 毫升）下也未改变正常的卵裂。
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