Urrea D A, Carranza J C, Cuba C A Cuba, Gurgel-Gonçalves R, Guhl F, Schofield C J, Triana O, Vallejo G A
Laboratorio de Investigaciones en Parasitología Tropical, Facultad de Ciencias, Universidad del Tolima, A.A. No. 546, Ibagué, Colombia.
Infect Genet Evol. 2005 Mar;5(2):123-9. doi: 10.1016/j.meegid.2004.07.005.
We present data on the molecular characterisation of strains of Trypanosoma rangeli isolated from naturally infected Rhodnius ecuadoriensis in Peru, from Rhodnius colombiensis, Rhodnius pallescens and Rhodnius prolixus in Colombia, and from Rhodnius pallescens in Panama. Strain characterisation involved a duplex PCR with S35/S36/KP1L primers. Mini-exon gene analysis was also carried out using TrINT-1/TrINT-2 oligonucleotides. kDNA and mini-exon amplification indicated dimorphism within both DNA sequences: (i) KP1, KP2 and KP3 or (ii) KP2 and KP3 products for kDNA, and 380 bp or 340 bp products for the mini-exon. All T. rangeli strains isolated from R. prolixus presented KP1, KP2 and KP3 products with the 340 bp mini-exon product. By contrast, all T. rangeli strains isolated from R. ecuadoriensis, R. pallescens and R. colombiensis, presented profiles with KP2 and KP3 kDNA products and the 380 bp mini-exon product. Combined with other studies, these results provide evidence of co-evolution of T. rangeli strains associated with different Rhodnius species groups east and west of the Andean mountains.
我们展示了从秘鲁自然感染的厄瓜多尔红猎蝽、哥伦比亚的哥伦比亚红猎蝽、浅色红猎蝽和长红猎蝽以及巴拿马的浅色红猎蝽中分离出的克氏锥虫菌株的分子特征数据。菌株鉴定采用S35/S36/KP1L引物进行双重PCR。还使用TrINT-1/TrINT-2寡核苷酸进行了小外显子基因分析。kDNA和小外显子扩增表明两个DNA序列内存在二态性：(i) kDNA的KP1、KP2和KP3或(ii) KP2和KP3产物，小外显子的产物为380 bp或340 bp。从长红猎蝽分离出的所有克氏锥虫菌株均呈现KP1、KP2和KP3产物以及340 bp的小外显子产物。相比之下，从厄瓜多尔红猎蝽、浅色红猎蝽和哥伦比亚红猎蝽分离出的所有克氏锥虫菌株均呈现KP2和KP3 kDNA产物以及380 bp的小外显子产物。结合其他研究，这些结果为安第斯山脉东西两侧与不同红猎蝽物种组相关的克氏锥虫菌株的共同进化提供了证据。
