Carpenter A, Purdy W C
Department of Chemistry, McGill University, Montreal, Quebec, Canada.
J Chromatogr. 1992 Jan 3;573(1):132-5. doi: 10.1016/0378-4347(92)80485-9.
The preparation of a stable affinity medium with heparin as the affinity ligand has been investigated. Glyceryl controlled-pore glass (CPG) was activated with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate and coupled with heparin. This affinity medium was used to separate some simple proteins, trypsinogen and lysozyme, in a high-performance liquid chromatographic configuration. The heparin-glyceryl-CPG was also used to separate alpha- and beta-lipoproteins in human serum. The effectiveness of the separation is confirmed by radial immunodiffusion and the determination of the cholesterol content of each of the separated fractions.
