Crone H D, Dawson R M
J Chromatogr. 1976 Dec 22;129:91-6. doi: 10.1016/s0021-9673(00)87771-7.
A commercial controlled-pore glass medium for exclusion chromatography which is substituted with glycerol to eliminate non-specific adsorption (Glyceryl-CPG) was examined. Experiments on the elution of acetylcholinesterase and ganglioside micelles at varied ionic strength and pH showed that a slight anionic character still persisted on the glass matrix. At ionic strengths above 0.1, this had no effect on the elution of proteins. The material was found to have no tendency to adsorb proteins and other compounds, and was judged an excellent medium for exclusion chromatography. As a support for affinity chromatography, Glyceryl-CPG could be activated by periodate to form a virtually neutral matrix-ligand system.
研究了一种用于排阻色谱的商业可控孔径玻璃介质，该介质用甘油取代以消除非特异性吸附（甘油基-CPG）。在不同离子强度和pH值下对乙酰胆碱酯酶和神经节苷脂胶束洗脱的实验表明，玻璃基质上仍存在轻微的阴离子特性。在离子强度高于0.1时，这对蛋白质的洗脱没有影响。发现该材料没有吸附蛋白质和其他化合物的倾向，被认为是排阻色谱的优良介质。作为亲和色谱的载体，甘油基-CPG可以用过碘酸盐活化，形成几乎中性的基质-配体系统。
