Tsfasman I M, Starovoĭtov I I, Ziakun A M, Skriabin G K
Biokhimiia. 1976 May;41(5):864-8.
Crude enzymatic preparation has been obtained from Pseudomonas bacteria which oxidises 1,8-DMN during 10-hour incubation with the following formation of the same products which are formed when this compound is oxidized by the intact cells. The first product of the oxidation is 1-methyl-8-oxymethylnaphtalene (compound I), obtained as a result of hydroxylation of one methyl group. Probably hydroxylase of 1,8-DMN may be referred to the class of oxigenases of the basis of the absence of 18O incorporation from H218O to compound I, and also resulting from the data on absorption of molecular oxygen during the reaction. The enzyme is completely inhibited by chelating agents of Fe2+ NAD(P)H and Fe2+ stimulates the reaction of 1.8 DMN oxidation.
已从假单胞菌属细菌中获得粗酶制剂，该制剂在10小时的孵育过程中氧化1,8 - 二甲基萘，并形成与该化合物被完整细胞氧化时相同的产物。氧化的第一个产物是1 - 甲基 - 8 - 羟甲基萘（化合物I），它是一个甲基羟基化的结果。由于没有从H₂¹⁸O中掺入¹⁸O到化合物I中，并且根据反应过程中分子氧吸收的数据，1,8 - 二甲基萘的羟化酶可能属于加氧酶类。该酶被Fe²⁺的螯合剂完全抑制，而NAD(P)H和Fe²⁺则刺激1,8 - 二甲基萘的氧化反应。
