Kumar R P, Streeleela N S, Rao P V, Vaidyanathan C S
J Bacteriol. 1973 Mar;113(3):1213-6. doi: 10.1128/jb.113.3.1213-1216.1973.
Evidence was obtained for the participation of iron in the double hydroxylation reaction catalyzed by anthranilate hydroxylase from Aspergillus niger (UBC 814). Omission of iron from the growth medium gave inactive preparations of anthranilate hydroxylase which could be reactivated by incubating the enzyme preparations with ferric citrate. The enzyme was susceptible to inhibition by metal chelating agents. The K(i) for o-phenanthroline, which inhibited the enzyme activity non-competitively with respect to anthranilate, was calculated to be 0.9 mM. The inhibition by o-phenanthroline was counteracted by ferric complexes such as ferric-ethylenediaminetetraacetic acid and ferric citrate. Anthranilate afforded protection against inhibition by o-phenanthroline.
已获得铁参与黑曲霉（UBC 814）邻氨基苯甲酸羟化酶催化的双羟基化反应的证据。从生长培养基中去除铁会得到无活性的邻氨基苯甲酸羟化酶制剂，通过将酶制剂与柠檬酸铁孵育可使其重新激活。该酶易受金属螯合剂的抑制。邻菲罗啉对酶活性的抑制作用相对于邻氨基苯甲酸是非竞争性的，其K(i)经计算为0.9 mM。邻菲罗啉的抑制作用可被铁络合物如铁 - 乙二胺四乙酸和柠檬酸铁抵消。邻氨基苯甲酸可提供针对邻菲罗啉抑制作用的保护。
