Cuartas Jaison H, Alzate Juan F, Moreno-Herrera Claudia X, Marquez Edna J
Facultad de Ciencias, Laboratorio de Biología Molecular y Celular, Universidad Nacional de Colombia, Medellín, Antioquia, Colombia.
Facultad de Medicina, Centro Nacional de Secuenciación Genómica, Universidad de Antioquia, Medellín, Antioquia, Colombia.
PeerJ. 2018 Feb 15;6:e4307. doi: 10.7717/peerj.4307. eCollection 2018.
The endangered marine gastropod, is an important fishery resource in the Caribbean region. Microbiological and parasitological research of this species have been poorly addressed despite its role in ecological fitness, conservation status and prevention of potential pathogenic infections. This study identified taxonomic groups associated with orange colored protrusions in the muscle of queen conchs using histological analysis, 454 pyrosequencing, and a combination of PCR amplification and automated Sanger sequencing. The molecular approaches indicate that the etiological agent of the muscle protrusions is a parasite belonging to the subclass Digenea. Additionally, the scope of the molecular technique allowed the detection of bacterial and fungi clades in the assignment analysis. This is the first evidence of a digenean infection in the muscle of this valuable Caribbean resource.
这种濒危的海洋腹足纲动物是加勒比地区重要的渔业资源。尽管该物种在生态适应性、保护状况以及预防潜在病原体感染方面具有重要作用，但对其进行的微生物学和寄生虫学研究却很少。本研究使用组织学分析、454焦磷酸测序以及PCR扩增与自动桑格测序相结合的方法，确定了与皇后海螺肌肉中橙色突起相关的分类群。分子生物学方法表明，肌肉突起的病原体是一种属于复殖亚纲的寄生虫。此外，分子技术的应用范围使得在分类分析中能够检测到细菌和真菌类群。这是这种珍贵的加勒比资源肌肉中存在复殖吸虫感染的首个证据。
