Lai S C, Chen K M, Chen H C, Lee H H
Department of Parasitology, Chung Shan Medical University, 110, Section 1, Chien-Kuo North Road, 402, Taichung , Taiwan.
Parasitol Res. 2005 Feb;95(3):193-200. doi: 10.1007/s00436-004-1271-9. Epub 2005 Jan 6.
The relationships between inflammation in organs with Toxocara canis larval migration and matrix metalloproteinase-9 (MMP-9) were investigated following the infection of mice with 1,000 infective eggs. Gelatinase activity was defined by gelatin zymography, optimum pH, inhibitor specificity and Western blot analysis. MMP-9 activity was present in the lungs, liver, muscles, and brain during T. canis larval migration. This enzyme had a molecular weight of about 94 kDa and showed maximum activity in the pH range of 6-8. The increased MMP-9 proteinases coincided with larval recovery and the degree of inflammation among the four organs. These results suggest that MMP-9 may be associated with the inflammatory reaction to larval toxocariasis during early migration, and may therefore be a useful marker during T. canis larvae migration.
在用1000个感染性虫卵感染小鼠后，研究了犬弓首线虫幼虫移行器官中的炎症与基质金属蛋白酶-9（MMP-9）之间的关系。通过明胶酶谱法、最适pH值、抑制剂特异性和蛋白质印迹分析来确定明胶酶活性。在犬弓首线虫幼虫移行期间，MMP-9活性存在于肺、肝、肌肉和脑中。这种酶的分子量约为94 kDa，在pH值6-8范围内表现出最大活性。MMP-9蛋白酶增加与幼虫恢复以及四个器官中的炎症程度一致。这些结果表明，MMP-9可能与早期移行期间对幼虫弓首线虫病的炎症反应有关，因此可能是犬弓首线虫幼虫移行期间的一个有用标志物。
