Echeverri Carolina, Fisher Stephen, King David, Craig Fiona E
Department of Pathology, University of Texas Health Science Center, San Antonio, USA.
Am J Clin Pathol. 2002 Apr;117(4):615-20. doi: 10.1309/AAYH-1FK8-38PL-Q6DT.
Flow cytometric analysis is important in the diagnosis, classification, and follow-up of non-Hodgkin lymphoma. It is assumed that the lymphoma phenotype for each patient remains unchanged over time and is consistent from one specimen to another. To determine the variability in expression of lymphoid antigens, we reviewed 211 flow cytometry specimens of malignant lymphoma from 81 patients. Some antigens showed a stable pattern of expression such as CD5, CD10, CD19, CD20, and HLA-DR. In contrast, CD21, CD22, CD23, and CD25 showed more variability from one specimen to another. We believe several factors affect the stability of antigen expression. True differences in expression most probably are related to the biology and function of the different antigens. For instance, CD19 and CD20 are essential in cell maturation and function and, therefore, are present on the majority of cells. In contrast, CD22 has a role during B-cell activation and, therefore, is more variable. Lack of standardization inflow cytometry procedures also is responsible for some variability. Instrument settings for adequate compensation and the criteria used to determine when an antigen is reported as positive are important considerations when evaluating flow cytometry histograms.
流式细胞术分析在非霍奇金淋巴瘤的诊断、分类及随访中具有重要意义。一般认为，每位患者的淋巴瘤表型随时间保持不变，且在不同标本间具有一致性。为确定淋巴样抗原表达的变异性，我们回顾了来自81例患者的211份恶性淋巴瘤流式细胞术标本。一些抗原呈现出稳定的表达模式，如CD5、CD10、CD19、CD20和HLA-DR。相比之下，CD21、CD22、CD23和CD25在不同标本间的变异性更大。我们认为有几个因素会影响抗原表达的稳定性。表达上的真正差异很可能与不同抗原的生物学特性和功能有关。例如，CD19和CD20在细胞成熟和功能中至关重要，因此存在于大多数细胞上。相比之下，CD22在B细胞激活过程中发挥作用，因此变异性更大。流式细胞术操作缺乏标准化也导致了一些变异性。在评估流式细胞术直方图时，用于适当补偿的仪器设置以及确定抗原何时报告为阳性的标准是重要的考虑因素。
