Burda Sherri T, Konings Frank A J, Williams Constance A U, Anyangwe Christopher, Nyambi Phillipe N
Department of Pathology, New York University School of Medicine, New York, New York 10016, USA.
AIDS Res Hum Retroviruses. 2004 Dec;20(12):1358-63. doi: 10.1089/aid.2004.20.1358.
This study describes the HIV-1 genetic diversity that currently circulates in Bamenda, the provincial capital of the North West province of Cameroon. Phylogenetic analysis of the protease (pro) gene of 20 HIV-1-seropositive individuals identified 11 (55%) CRF02_AG, one D, one F2, one J, and four (20%) unclassifiable strains. Interestingly, the remaining two (10%) samples, 02CMNYU3072 and 03CMNYU3224, originating from epidemiologically unlinked individuals, were classified as CRF09_cpx, representing the first reported cases of this complex circulating recombinant form (CRF) in Cameroon. Additional analysis of the C2V5 portion of the envelope (env) gene confirmed the CRF09_cpx identity of these isolates and classified the remaining isolates as CRF02_AG (n = 12, 63%), subtype D (n = 2, 11%), subtype F2 (n = 2, 11%), and subtype A1 (n = 1). In combination, the pro and env subtyping results revealed three (16%) isolates with discordant subtypes including J( pro )CRF02_AG( env ), CRF02_AG( pro )D( env ), and CRF02_AG( pro )F2( env ). In conclusion, this study highlights the presence of HIV-1 CRF09_cpx in Cameroon and identifies three possible intersubtype recombinants (ISRs) containing CRF02_AG in a town where CRF02_AG infections predominate, and stresses the commonness of HIV-1 recombinant strains in a region where broad genetic diversity exists.
本研究描述了目前在喀麦隆西北省省会巴门达流行的HIV-1基因多样性。对20名HIV-1血清阳性个体的蛋白酶（pro）基因进行系统发育分析，鉴定出11株（55%）CRF02_AG、1株D型、1株F2型、1株J型和4株（20%）无法分类的毒株。有趣的是，另外两个（10%）样本，即02CMNYU3072和03CMNYU3224，来自流行病学上无关联的个体，被归类为CRF09_cpx，这是喀麦隆首次报告的这种复杂循环重组形式（CRF）的病例。对包膜（env）基因C2V5部分的进一步分析证实了这些分离株为CRF09_cpx，并将其余分离株分类为CRF02_AG（n = 12，63%）、D亚型（n = 2，11%）、F2亚型（n = 2，11%）和A1亚型（n = 1）。综合来看，pro和env亚型分析结果显示有3株（16%）分离株的亚型不一致，包括J（pro）CRF02_AG（env）、CRF02_AG（pro）D（env）和CRF02_AG（pro）F2（env）。总之，本研究突出了喀麦隆存在HIV-1 CRF09_cpx，并在一个以CRF02_AG感染为主的城镇中鉴定出三种可能含有CRF02_AG的亚型间重组体（ISR），强调了在一个存在广泛基因多样性的地区HIV-1重组毒株的普遍性。
