沉默靠近tRNA基因的区域需要核仁定位。
Silencing near tRNA genes requires nucleolar localization.
作者信息
Wang Li, Haeusler Rebecca A, Good Paul D, Thompson Martin, Nagar Sapna, Engelke David R
机构信息
Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109-0606, USA.
出版信息
J Biol Chem. 2005 Mar 11;280(10):8637-9. doi: 10.1074/jbc.C500017200. Epub 2005 Jan 15.
Abstract
Transcription by RNA polymerase II is antagonized by the presence of a nearby tRNA gene in Saccharomyces cerevisiae. To test hypotheses concerning the mechanism of this tRNA gene-mediated (tgm) silencing, the effects of specific gene deletions were determined. The results show that the mechanism of silencing near tRNA genes is fundamentally different from other forms of transcriptional silencing in yeast. Rather, tgm silencing is dependent on the ability to cluster the dispersed tRNA genes in or near the nucleolus, constituting a form of three-dimensional gene control.
摘要
在酿酒酵母中，RNA聚合酶II的转录会受到附近tRNA基因的拮抗作用。为了检验关于这种tRNA基因介导（tgm）沉默机制的假设，我们确定了特定基因缺失的影响。结果表明，tRNA基因附近的沉默机制与酵母中其他形式的转录沉默根本不同。相反，tgm沉默依赖于将分散的tRNA基因聚集在核仁内或核仁附近的能力，构成了一种三维基因控制形式。
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