适体活性的光调控：一种带有笼蔽胸腺嘧啶核苷碱基的抗凝血酶适体。
Light regulation of aptamer activity: an anti-thrombin aptamer with caged thymidine nucleobases.
作者信息
Heckel Alexander, Mayer Günter
机构信息
Kekulé-Institute for Organic Chemistry and Biochemistry, University of Bonn, Gerhard-Domagk-Str. 1, 53121 Bonn, Germany.
出版信息
J Am Chem Soc. 2005 Jan 26;127(3):822-3. doi: 10.1021/ja043285e.
"Caged" derivatives of a 15 nucleotide ssDNA anti-thrombin aptamer have been synthesized in which thymidine nucleotides are modified with photolabile protecting groups. One caged thymidine in a key location is enough to completely mask the aptamer's function in respect to their affinity for thrombin and their inhibition of the blood clotting cascade. With light (366 nm) the caging group can be removed, yielding the unmodified active aptamer.
已合成了一种15个核苷酸的单链DNA抗凝血酶适体的“笼化”衍生物，其中胸腺嘧啶核苷酸用光不稳定保护基团进行了修饰。在关键位置的一个笼化胸腺嘧啶就足以完全掩盖适体对凝血酶的亲和力及其对血液凝固级联反应的抑制作用方面的功能。用光（366纳米）可以去除笼化基团，得到未修饰的活性适体。
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