Nechaeva Marina V, Makarenko Irina G, Tsitrin Evgenii B, Zhdanova Nadezhda P
Laboratory of General Physiology, Institute of Developmental Biology RAS, Vavilov Str. 26, Moscow 119334, Russia.
Comp Biochem Physiol A Mol Integr Physiol. 2005 Jan;140(1):19-28. doi: 10.1016/j.cbpb.2004.09.024.
A morpho-functional study of the veiled chameleon (Chamaeleo calyptratus) amnion was performed as part of a general comparative study of amnion rhythmic contractions (ARC) in reptile and bird embryogenesis. Eggs incubated at 27.5 degrees C were used at different developmental stages from day 80 (D80) to D184 for the recording of ARC using a force transducer. Slow ARC, about 1 min in duration, were revealed from D88 (stage 31) to the near-hatching stages (incubation time was 183-198 days). The frequency and amplitude of slow ARC increased significantly towards the end of incubation. This ARC enhancement correlated with the differentiation of smooth muscle elements in the amniotic membrane from single spindle-shaped cells to complex "star-like" structures and with the development of the actin fibers, revealed by phalloidin, in the amniotic muscle layer. Short-term changes in temperature influenced ARC and heart rate (HR). Cooling to 25 degrees C from the control (27.5 degrees C) resulted in a significant decrease in both ARC frequency and HR. Heating to 30 degrees C significantly increased the embryonic HR, but not ARC frequency. Within the temperature range from 25 to 30 degrees C the temperature effect on ARC frequency and HR was reversible.
作为对爬行动物和鸟类胚胎发育过程中羊膜节律性收缩（ARC）进行的一项综合比较研究的一部分，对蒙面避役（Chamaeleo calyptratus）的羊膜进行了形态功能研究。使用在27.5摄氏度下孵化的卵，在从第80天（D80）到第184天的不同发育阶段，使用力传感器记录ARC。从第88天（第31阶段）到接近孵化阶段（孵化时间为183 - 198天）发现了持续时间约为1分钟的缓慢ARC。在孵化末期，缓慢ARC的频率和幅度显著增加。这种ARC增强与羊膜中平滑肌成分从单个纺锤形细胞分化为复杂的“星状”结构以及用鬼笔环肽显示的羊膜肌层中肌动蛋白纤维的发育相关。温度的短期变化影响ARC和心率（HR）。从对照温度（27.5摄氏度）冷却至25摄氏度导致ARC频率和HR均显著下降。加热至30摄氏度显著增加胚胎心率，但不增加ARC频率。在25至30摄氏度的温度范围内，温度对ARC频率和HR的影响是可逆的。
