大鼠尿液中的新木脂素代谢产物。

New lignan metabolites in rat urine.

作者信息

Smeds Annika I, Saarinen Niina M, Eklund Patrik C, Sjöholm Rainer E, Mäkelä Sari I

机构信息

Department of Organic Chemistry, Abo Akademi University, Biskopsgatan 8, FIN-20500 Turku/Abo, Finland.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):87-97. doi: 10.1016/j.jchromb.2004.11.017.

DOI:10.1016/j.jchromb.2004.11.017

PMID:15664338

Abstract

Ten potential lignan metabolites were quantified in rat urine extracts using liquid chromatography-tandem mass spectrometry. The rats were orally administered with the plant lignans 7-hydroxymatairesinol, matairesinol, lariciresinol or secoisolariciresinol, or with the mammalian lignan enterolactone. The samples were enzymatically hydrolysed and solid-phase extracted before analysis. Of the analysed compounds, only trace amounts of 7-oxoenterolactone could be detected in the urine extracts before administration, but after administration of any of the lignans, the excretion of 7-oxoenterolactone increased and monodemethylated matairesinol and 4,4'-dihydroxyenterolactone could be detected. In addition, other novel lignan metabolites were detected, i.e., 7-oxomatairesinol, alpha-conidendrin, and alpha- and beta-conidendric acid.

摘要

使用液相色谱-串联质谱法对大鼠尿液提取物中的十种潜在木脂素代谢物进行了定量分析。给大鼠口服植物木脂素7-羟基落叶松脂素、落叶松脂素、松脂醇或开环异落叶松脂素，或哺乳动物木脂素肠内酯。在分析之前，对样品进行酶解和固相萃取。在分析的化合物中，给药前在尿液提取物中仅能检测到痕量的7-氧代肠内酯，但在给予任何一种木脂素后，7-氧代肠内酯的排泄增加，并且可以检测到单去甲基落叶松脂素和4,4'-二羟基肠内酯。此外，还检测到了其他新型木脂素代谢物，即7-氧代落叶松脂素、α-罗汉松脂酚以及α-和β-罗汉松脂酸。