甲醇塞辅助扫集胶束电动色谱法结合紫光发光二极管诱导荧光检测法测定尿液中的多巴胺

Methanol plug assisted sweeping-micellar electrokinetic chromatography for the determination of dopamine in urine by violet light emitting diode-induced fluorescence detection.

作者信息

Huang Hsuan-Ming, Lin Cheng-Huang

机构信息

Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Tingchow Road, Taipei, Taiwan.

出版信息

J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Feb 25;816(1-2):113-9. doi: 10.1016/j.jchromb.2004.11.018.

DOI:10.1016/j.jchromb.2004.11.018

PMID:15664341

Abstract

The use and limitations of a methanol plug assisted sweeping-micellar electrokinetic chromatography (sweeping-MEKC) method is described. Using naphthalene-2,3-dicarboxaldehyde (NDA)-labeled dopamine as a model compound, this new method was also used in the determination of dopamine in actual urine samples. An inexpensive violet light emitting diode (LED) was used for the light source, because this is suitable for fluorescence excitation. The number of theoretical plates of the analyte was determined to be approximately 1 x 10(5) and approximately 2 x 10(5) by means of MEKC and sweeping-MEKC and this was improved to approximately 1 x 10(6) when the methanol plug assisted mode was applied. In addition, the detection limit of NDA-labeled dopamine was determined to be 9.1 x 10(-7) and 1.2 x 10(-8)M by means of MEKC and sweeping-MEKC and this was improved to 4.7 x 10(-9)M when the methanol plug assisted sweeping-MEKC mode was applied.

摘要

描述了甲醇塞辅助扫集-胶束电动色谱法（扫集-MEKC）的使用及局限性。以萘-2,3-二甲醛（NDA）标记的多巴胺作为模型化合物，该新方法还用于实际尿液样本中多巴胺的测定。使用廉价的紫光发光二极管（LED）作为光源，因为它适用于荧光激发。通过MEKC和扫集-MEKC测定，分析物的理论塔板数分别约为1×10⁵和约2×10⁵，当采用甲醇塞辅助模式时，理论塔板数提高到约1×10⁶。此外，通过MEKC和扫集-MEKC测定，NDA标记多巴胺的检测限分别为9.1×10⁻⁷和1.2×10⁻⁸M，当采用甲醇塞辅助扫集-MEKC模式时，检测限提高到4.7×10⁻⁹M。