采用动态pH连接技术结合毛细管电泳/蓝光发光二极管（LED）诱导荧光检测法分析啤酒中的核黄素。

Analysis of riboflavin in beer by capillary electrophoresis/blue light emitting diode (LED)-induced fluorescence detection combined with a dynamic pH junction technique.

作者信息

Su An-Kai, Chang Yen-Sheng, Lin Cheng-Huang

机构信息

Department of Chemistry, National Taiwan Normal University, 88 Sec. 4, Tingchow Road, Taipei, Taiwan.

出版信息

Talanta. 2004 Nov 15;64(4):970-4. doi: 10.1016/j.talanta.2004.04.011.

DOI:10.1016/j.talanta.2004.04.011

PMID:18969698

Abstract

A simple, inexpensive and reliable method for the routine analysis of riboflavin in beer by capillary electrophoresis-light emitting diode (CE-LED) induced fluorescence detection is described. A simple and straightforward sample preparation is involved and the method is based on an inexpensive blue LED as the light source combined with an on-line sample concentration technique. For this detection system, using a normal micellar electrokinetic chromatography (MEKC), stacking-MEKC and dynamic pH junction techniques, the detection limits were found to be 480, 20 and 1ngmL(-1), respectively. In addition, the number of theoretical plates for riboflavin was determined to be 3.8x10(4) by means of a dynamic pH junction and this was improved to 3.2x10(6) when the dynamic pH junction-sweeping mode was applied. The concentrations of riboflavin in 12 samples of different types of commercial beer were found to be in the range of 130-280ngmL(-1).

摘要

本文描述了一种采用毛细管电泳 - 发光二极管（CE - LED）诱导荧光检测法对啤酒中核黄素进行常规分析的简单、廉价且可靠的方法。该方法涉及简单直接的样品制备，基于廉价的蓝色发光二极管作为光源，并结合在线样品浓缩技术。对于此检测系统，采用常规胶束电动色谱（MEKC）、堆积 - MEKC和动态pH连接技术时，检测限分别为480、20和1 ng mL⁻¹。此外，通过动态pH连接法测定核黄素的理论塔板数为3.8×10⁴，当应用动态pH连接 - 扫集模式时，理论塔板数提高到3.2×10⁶。在12种不同类型的市售啤酒样品中，核黄素的浓度范围为130 - 280 ng mL⁻¹。