Bayat Mohammad, Vasheghani Mohammad Mehdi, Razavi Nasser, Taheri Sudabeh, Rakhshan Mohammad
Anatomy Department, Medical Faculty, Cell and Molecular Biology Research Center, Shaheed Beheshti University of Medical Sciences, P.O. Box 19395/4719, Tehran, Iran.
J Photochem Photobiol B. 2005 Feb 1;78(2):171-7. doi: 10.1016/j.jphotobiol.2004.08.012.
This paper presents the results of a study on the effects of two different doses of low-level laser therapy on healing of deep second-degree burns. Sixty rats were randomly allocated to one of four groups. A deep second-degree burn was inflicted in each rat. In the control group burns remained untreated; in two laser treated groups the burns were irradiated daily with low-level helium-neon laser with energy densities of 1.2 and 2.4 J/cm2, respectively. In the fourth group the burns were treated topically with 0.2% nitrofurazone cream every day. The response to treatments was assessed histologically at 7, 16 and 30 days after burning, and microbiologically at Day 15. The number of macrophages at day 16, and the depth of new epidermis at day 30, was significantly less in the laser treated groups in comparison with control and nitrofurazone treated groups (P=0.000). Staphylococcus epidermidis was found in the 70% of rat wounds in the laser treated groups in comparison with 100% of rats in the control group. S. aureus was found in the 40% rat wounds in the nitrofurazone treated group, but there was not found in the wounds of laser treated, and control groups. It is concluded that low-level laser therapy of deep second-degree burn caused significant decrease in the number of macrophage and depth of new epidermis. In addition, it decreased incidence of S. epidermidis and S. aureus.
本文介绍了一项关于两种不同剂量的低强度激光疗法对深二度烧伤愈合影响的研究结果。60只大鼠被随机分为四组。每只大鼠造成深二度烧伤。对照组烧伤不予治疗；在两个激光治疗组中，烧伤部位分别每天用能量密度为1.2和2.4 J/cm²的低强度氦氖激光照射。第四组每天用0.2%呋喃西林乳膏局部治疗烧伤。在烧伤后7天、16天和30天进行组织学评估治疗反应，在第15天进行微生物学评估。与对照组和呋喃西林治疗组相比，激光治疗组在第16天的巨噬细胞数量和第30天的新表皮深度明显减少（P = 0.000）。激光治疗组70%的大鼠伤口中发现表皮葡萄球菌，而对照组为100%。呋喃西林治疗组40%的大鼠伤口中发现金黄色葡萄球菌，但激光治疗组和对照组的伤口中未发现。结论是，深二度烧伤的低强度激光治疗导致巨噬细胞数量和新表皮深度显著减少。此外，它降低了表皮葡萄球菌和金黄色葡萄球菌的发生率。
