Solid Tumor Research Center, Department of Medical Physics and Imaging, Urmia University of Medical Sciences, Urmia, Iran.
Neurophysiology Research Center, Department of Anatomy, Urmia University of Medical Sciences, Urmia, Iran.
Kaohsiung J Med Sci. 2017 Aug;33(8):385-393. doi: 10.1016/j.kjms.2017.05.013. Epub 2017 Jul 1.
A recent hypothesis has revealed that low-dose irradiation (LDI) with ionizing radiation might have a promoting effect on fracture healing. The aim of this study was to investigate the influence of direct (electron beam) and indirect (gamma-ray) low-dose ionizing irradiations on the wound healing process in male rats. In 72 male rats, a full-thickness wound was incised. The animals were randomly assigned to three groups, each with 24 rats. The first two groups were named IG-I and IG-II and respectively exposed to electron and gamma-radiations (75 cGy) immediately after the surgical procedure. The third group was considered as the control (CG) and remained untreated. Skin biopsies from the subgroups were collected on days 3, 7, 15, and 21 after the operation and evaluated using histological and biomechanical methods. Data were analyzed by one-way ANOVA, followed by Tukey's post hoc test using SPSS 20 software. Histological studies of tissues showed that the mean number of fibroblasts, macrophages, blood vessel sections, and neutrophils on the third and seventh days after the surgery in the gamma-treated group was higher than that in both other groups. In contrast, on day 21, the mean number of mentioned cells in the gamma-treated group was lower than in the other two groups. In addition, the mean maximum stress value was significantly greater in the gamma-treated group. Results of this study showed that gamma-ray irradiation is effective in the acceleration of wound healing.
最近的一个假设表明，低剂量辐射（LDI）可能对骨折愈合有促进作用。本研究旨在探讨直接（电子束）和间接（伽马射线）低剂量电离照射对雄性大鼠伤口愈合过程的影响。在 72 只雄性大鼠中，进行了全层切口。动物被随机分为三组，每组 24 只。前两组分别命名为 IG-I 和 IG-II，并在手术后立即接受电子和伽马射线（75 cGy）照射。第三组被认为是对照组（CG），不进行任何治疗。术后第 3、7、15 和 21 天，从小鼠皮肤活检中收集组织，并采用组织学和生物力学方法进行评估。使用 SPSS 20 软件进行单因素方差分析，然后进行 Tukey 事后检验。组织学研究表明，手术后第 3 天和第 7 天，伽马处理组的成纤维细胞、巨噬细胞、血管切片和中性粒细胞的平均数量高于其他两组。相比之下，在第 21 天，伽马处理组上述细胞的平均数量低于其他两组。此外，伽马处理组的最大应力值明显更大。本研究结果表明，伽马射线照射可加速伤口愈合。
