Karplus Martin, Gao Yi Qin, Ma Jianpeng, van der Vaart Arjan, Yang Wei
Laboratoire de Chimie Biophysique, ISIS, Université Louis Pasteur, Strasbourg, France.
Philos Trans A Math Phys Eng Sci. 2005 Feb 15;363(1827):331-55; discussion 355-6. doi: 10.1098/rsta.2004.1496.
Living cells are a collection of molecular machines which carry out many of the functions essential for the cell's existence, differentiation and reproduction. Most, though not all, of these machines are made up of proteins. Because of their complexity, an understanding of how they work requires a synergistic combination of experimental and theoretical studies. In this paper we outline our studies of two such protein machines. One is GroEL, the chaperone from Escherichia coli, which aids in protein folding; the other is F(1)-ATPase, a motor protein which synthesizes and hydrolyses ATP.
活细胞是由分子机器组成的集合体，这些分子机器执行着许多对细胞的生存、分化和繁殖至关重要的功能。这些机器中的大多数（尽管不是全部）由蛋白质构成。由于它们的复杂性，要了解它们如何工作需要实验研究和理论研究的协同结合。在本文中，我们概述了对两种这样的蛋白质机器的研究。一种是来自大肠杆菌的伴侣蛋白GroEL，它有助于蛋白质折叠；另一种是F(1)-ATP酶，一种合成和水解ATP的驱动蛋白。
