[念珠菌和曲霉菌抗体酶联免疫吸附测定——临床真菌学常规诊断血清中预期的抗体阳性结果]

[Candida- and Aspergillus-antibody-Elisa--expected positive antibody results in sera of the clinical-mycological routine diagnostics].

作者信息

Fegeler W, Kipp F

机构信息

Institut für Medizinische Mikrobiologie, Universitätsklinikum Münster, Westfälische Wilhelms-Universität Münster, Domagkstrasse 10, D-48149 Münster, Germany.

出版信息

Mycoses. 2004;47 Suppl 1:41-7. doi: 10.1111/j.1439-0507.2004.01033.x.

DOI:10.1111/j.1439-0507.2004.01033.x

PMID:15667364

Abstract

Based on the ELISA results of more than 15000 serum samples of clinical mycological routine diagnostics, the expected frequency of positive antibody ELISA results within the immunoglobulin classes IgM, IgG and IgA was determined, to optimize the diagnostic assessment of first or single result of Candida or Aspergillus antibody ELISA. In general diagnostics the expected frequency of positive antibody ELISA results of the first sample within the immunoglobulin classes were as follows: Candida antibody IgM 6.1%; IgG 6.0%; IgA 2.1% and Aspergillus antibody IgM 11.4%; IgG 22.1% and IgA 5.1%, respectively. Using the Candida antibody ELISA as confirmation test only, percentages of positive antibody results in the first sample were 2.5 to 3 times higher than in general diagnostics. In follow-up examinations the Candida antibodies showed different kinetics within the immunoglobulin classes compared to those of the Aspergillus antibodies.

摘要

基于15000多份临床真菌学常规诊断血清样本的酶联免疫吸附测定（ELISA）结果，确定了免疫球蛋白类别IgM、IgG和IgA中抗体ELISA阳性结果的预期频率，以优化念珠菌或曲霉抗体ELISA首次或单次结果的诊断评估。在一般诊断中，免疫球蛋白类别中首个样本抗体ELISA阳性结果的预期频率如下：念珠菌抗体IgM为6.1%；IgG为6.0%；IgA为2.1%，曲霉抗体IgM为11.4%；IgG为22.1%；IgA为5.1%。仅将念珠菌抗体ELISA用作确认试验时，首个样本中抗体阳性结果的百分比比一般诊断高2.5至3倍。在后续检查中，与曲霉抗体相比，念珠菌抗体在免疫球蛋白类别中表现出不同的动力学。