Komoda Teruko
Department of Medical Technology, Faculty of Health Sciences, Kyorin University, Tokyo 192-8508, Japan.
Jpn J Infect Dis. 2007 Nov;60(6):347-51.
To assess the importance of only IgA antibody positivity in the peptide-based ELISA (P-ELISA) examination of kinetic behaviors of antibodies (IgA, IgG) to Chlamydia trachomatis, 426 sera from 52 follow-up antigen-positive patients were assayed. In part, a microimmunofluorescence (MIF) test and an immunoblot (IB) assay were also used for confirmation. The results showed that the positivity rates of IgA and IgG antibodies were 82.7 and 96.2%, respectively, at the first testing. One patient had both IgA- and IgG-negative antibodies at the first testing, but this became only IgA-positive and then IgG-positive. The patient was co-infected with Candida albicans and C. trachomatis, and saw a gynecologist for the symptom of itching. Although the major outer membrane protein was negative in IB assay, the results of the MIF test and absorption experiments were positive. MIF titers for IgA and IgG antibodies to C. pneumoniae were <1:8 and 1:32, respectively, at the peak level of P-ELISA. These findings seem to suggest that when only the IgA antibody is detected by P-ELISA, C. trachomatis infection may be present at an early stage, so confirmation via testing for C. trachomatis is needed.
为评估在基于肽的酶联免疫吸附测定（P-ELISA）检测沙眼衣原体抗体（IgA、IgG）动力学行为时仅IgA抗体阳性的重要性，对52例随访抗原阳性患者的426份血清进行了检测。部分检测还采用了微量免疫荧光（MIF）试验和免疫印迹（IB）分析进行确认。结果显示，首次检测时IgA和IgG抗体的阳性率分别为82.7%和96.2%。1例患者首次检测时IgA和IgG抗体均为阴性，但之后仅IgA转为阳性，随后IgG也转为阳性。该患者同时感染了白色念珠菌和沙眼衣原体，因瘙痒症状就诊于妇科医生。尽管IB分析中主要外膜蛋白为阴性，但MIF试验和吸收实验结果为阳性。在P-ELISA的峰值水平时，针对肺炎衣原体的IgA和IgG抗体的MIF滴度分别<1:8和1:32。这些发现似乎表明，当通过P-ELISA仅检测到IgA抗体时，沙眼衣原体感染可能处于早期阶段，因此需要通过检测沙眼衣原体进行确认。
