[用于检测系统性念珠菌病中抗白色念珠菌IgG抗体的标准化免疫酶分析]

[Standardized immunoenzyme analysis for detection of IgG antibodies against Candida albicans in systemic candidiasis].

作者信息

Carceller A, Torres-Rodríguez J M, Lowinger M, Alía C

机构信息

Departamento de Microbiología, Institut Municipal d'Investigacions Mèdiques, Barcelona.

出版信息

Enferm Infecc Microbiol Clin. 1992 Apr;10(4):195-9.

PMID:1606221

Abstract

We evaluate the usefulness of an ELISA test for detecting IgG antibodies against Candida albicans somatic and metabolic antigens. We studied 68 sera samples of patients with disseminated candidosis due to C. albicans in most of the cases. We used an ELISA test standardized with a monoclonal anti-IgG conjugate marked with peroxidase and soluble C. albicans antigens. We used as controls 84 sera from healthy individuals or patients without any evidence for disseminated candidosis. The maximum sensitivity for protein antigen of metabolic origin was 67.6%, with an specificity of 77.3%. For the somatic antigen, specificity was higher (83.3%) and positive predictive value was 0.741. We believe that IgG-ELISA gives useful data for diagnosis and follow-up of patients with disseminated candidosis.

摘要

我们评估了一种酶联免疫吸附测定（ELISA）试验检测抗白色念珠菌体细胞和代谢抗原IgG抗体的实用性。在大多数病例中，我们研究了68例因白色念珠菌引起播散性念珠菌病患者的血清样本。我们使用了一种经标准化的ELISA试验，该试验采用了用辣根过氧化物酶标记的单克隆抗IgG缀合物和可溶性白色念珠菌抗原。我们将84例健康个体或无播散性念珠菌病证据患者的血清用作对照。代谢来源的蛋白质抗原的最大敏感性为67.6%，特异性为77.3%。对于体细胞抗原，特异性更高（83.3%），阳性预测值为0.741。我们认为IgG-ELISA可为播散性念珠菌病患者的诊断和随访提供有用的数据。