Burchiel Scott W, Lauer Fredine T, Dunaway Sandy L, Zawadzki Jerome, McDonald Jacob D, Reed Matthew D
College of Pharmacy Toxicology Program, The University of New Mexico, Albuquerque, NM 87131, USA.
Toxicol Appl Pharmacol. 2005 Feb 1;202(3):229-36. doi: 10.1016/j.taap.2004.06.024.
The purpose of these studies was to assess the effects of hardwood smoke (HWS) inhalation (30-1000 microg/m3) on the systemic immune responses of A/J mice evaluated after 6 months of daily exposures. Spleen cells obtained from mice were assessed for changes in cell number, cell surface marker expression [B, T, macrophage, and natural killer (NK) cells], and responses to B cell (LPS, endotoxin) and T cell (Con A) mitogens. Results showed that HWS smoke increased T cell proliferation in the 100 microg/m3 exposure group and produced a concentration-dependent suppression of T cell proliferation at concentrations >300 microg/m3. There were no effects on B cell proliferation or in spleen cell surface marker expression. Analyses of the exposure atmospheres revealed the presence of significant levels of naphthalene and methylated napthalenes, fluorene, phenanthrene, and anthracene in the exposure chambers, as well as low concentrations of several metals (K, Ca, and Fe). Our results demonstrate that environmentally relevant concentrations of HWS may be immunosuppressive to the immune system of mice exposed during a 6-month period.
这些研究的目的是评估吸入硬木烟雾（HWS，浓度为30 - 1000微克/立方米）对A/J小鼠全身免疫反应的影响，这些小鼠每天暴露，持续6个月后进行评估。对从小鼠获取的脾细胞进行细胞数量、细胞表面标志物表达（B细胞、T细胞、巨噬细胞和自然杀伤细胞）以及对B细胞（脂多糖、内毒素）和T细胞（刀豆蛋白A）促有丝分裂原反应的变化评估。结果显示，在100微克/立方米暴露组中，HWS烟雾增加了T细胞增殖，而在浓度>300微克/立方米时对T细胞增殖产生浓度依赖性抑制。对B细胞增殖或脾细胞表面标志物表达没有影响。对暴露环境的分析显示，暴露室内存在大量的萘和甲基萘、芴、菲和蒽，以及低浓度的几种金属（钾、钙和铁）。我们的结果表明，与环境相关浓度的HWS可能对在6个月期间暴露的小鼠免疫系统具有免疫抑制作用。
