Oligonucleotide conformations. (5) NMR and relaxation studies on GpU and UpG at neutral pH. - Suppr | 超能文献

Oligonucleotide conformations. (5) NMR and relaxation studies on GpU and UpG at neutral pH.

作者信息

Chachaty C, Yokono T, Tran-Dinh S, Guschlbauer W

出版信息

Biophys Chem. 1977 Jan;6(2):151-9. doi: 10.1016/0301-4622(77)87004-x.

DOI:10.1016/0301-4622(77)87004-x

PMID:15669

Abstract

The average conformation of GpU and UpG in neutral aqueous solutions has been investigated by proton chemical shifts and coupling measurements as well as T1 relaxation time experiments. The proportion of the N and S pseudorotational conformers of the ribose ring has been derived from the vicinal coupling constants. The relaxation data provide information about the syn--anti equilibrium of the orientation of the base about the glycosidic bond. This orientation is predominantly syn for the Guo base in both dinucleoside phosphates, that of Urd is anti in the case of GpU and shows an almost equivalent syn and anti character for UpG.

摘要

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