抗线粒体抗体及其亚型在原发性胆汁性肝硬化诊断中的价值

[The value of antimitochondrial antibody and its subtypes in the diagnosis of primary biliary cirrhosis].

作者信息

Yao Ding-Kang, Xie Wei-Fen, Chen Wei-Zhong, Liu Hai-Ying, Tu Xiao-Qing, Fan Li-Ying

机构信息

Department of Gastroenterology and Clinical Immunology Center, Chang Zheng Hospital, Second Military Medical University, Shanghai 200003, China.

出版信息

Zhonghua Gan Zang Bing Za Zhi. 2005 Jan;13(1):9-11.

PMID:15670482

Abstract

OBJECTIVE

A study on the value of antimitochondrial antibody (AMA) and its subtypes anti-M2, anti-M4, and anti-M9 in diagnosing primary biliary cirrhosis (PBC).

METHODS

Antimitochondrial antibody was detected by indirect immunofluorescence and anti-M2, anti-M4 and anti-M9 by Western blotting. AMA and anti-M2 of 78 PBC patients, of 35 non-PBC hepato-biliary disease patients and 20 healthy controls were studied and anti-M2, anti-M4 and anti-M9 were studied in 30 of the 78 PBC patients.

RESULTS

96.2% (75/78) of PBC patients were AMA positive and 94.9% (74/78) of PBC patients were anti-M2 positive. Only three among the 35 non-PBC patients were positive for AMA (one with very low titre). None of the 35 non-PBC patients was anti-M2 positive. AMA and anti-M2 were negative in all the healthy controls. Among the 30 anti-M2 positive patients, 16 patients were anti-M4 positive (16/30, 53.3%) and 4 patients were anti-M9 positive (4/30, 13.3%).

CONCLUSION

AMA and its subtypes (special anti-M2) are important sero-immunological markers for the diagnosis of PBC.

摘要

目的

研究抗线粒体抗体（AMA）及其亚型抗-M2、抗-M4和抗-M9在原发性胆汁性肝硬化（PBC）诊断中的价值。

方法

采用间接免疫荧光法检测抗线粒体抗体，采用蛋白质印迹法检测抗-M2、抗-M4和抗-M9。对78例PBC患者、35例非PBC肝胆疾病患者和20例健康对照者进行AMA和抗-M2检测，并对78例PBC患者中的30例进行抗-M2、抗-M4和抗-M9检测。

结果

PBC患者中96.2%（75/78）AMA阳性，94.9%（74/78）抗-M2阳性。35例非PBC患者中仅3例AMA阳性（1例滴度极低）。35例非PBC患者中无抗-M2阳性。所有健康对照者的AMA和抗-M2均为阴性。在30例抗-M2阳性患者中，16例抗-M4阳性（16/30，53.3%），4例抗-M9阳性（4/30，13.3%）。