缓冲溶液中和银岛膜上Cy5-DNA的双光子诱导荧光。
Two-photon induced fluorescence of Cy5-DNA in buffer solution and on silver island films.
作者信息
Lukomska Joanna, Gryczynski Ignacy, Malicka Joanna, Makowiec Slawomir, Lakowicz Joseph R, Gryczynski Zygmunt
机构信息
Center for Fluorescence Spectroscopy, Department of Biochemistry and Molecular Biology, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA.
出版信息
Biochem Biophys Res Commun. 2005 Mar 4;328(1):78-84. doi: 10.1016/j.bbrc.2004.12.148.
Abstract
We report the observation of a strong two-photon induced fluorescence emission of Cy5-DNA within the tunable range of a Ti:Sapphire laser. The estimated two-photon cross-section for Cy5-DNA of 400GM is about 3.5-fold higher than it was reported for rhodamine B. The fundamental anisotropies of Cy5-DNA are close to the theoretical limits of 2/5 and 4/7 for one- and two-photon excitation, respectively. We also observed an enhanced two-photon induced fluorescence (TPIF) of Cy5-DNA deposited on silver island films (SIFs). In the presence of SIFs, the TPIF is about 100-fold brighter. The brightness increase of Cy5-DNA TPIF near SIFs is mostly due to enhanced local field.
摘要
我们报告了在钛宝石激光器的可调谐范围内观察到Cy5-DNA的强双光子诱导荧光发射。估计Cy5-DNA的双光子截面为400GM，比报道的罗丹明B的双光子截面高约3.5倍。Cy5-DNA的基态各向异性分别接近单光子和双光子激发的理论极限2/5和4/7。我们还观察到沉积在银岛膜（SIFs）上的Cy5-DNA的双光子诱导荧光（TPIF）增强。在存在SIFs的情况下，TPIF亮约100倍。SIFs附近Cy5-DNA的TPIF亮度增加主要是由于局部场增强。
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