Mitsui Kazuhiro, Nakagawa Daichi, Nakamura Masahiro, Okamoto Taro, Tsurugi Kunio
University of Yamanashi, Faculty of Medicine, Department of Biochemistry 2, 1110 Shimokato, Tamaho, Nakakoma, Yamanashi 409-3898, Japan.
FEBS Lett. 2005 Jan 31;579(3):723-7. doi: 10.1016/j.febslet.2004.12.051.
Valproic acid (VPA) inhibited the growth of yeast in a dose-dependent manner with complete inhibition attained at 100 mM. When cells were exposed to 25 mM VPA, the wild-type died showing apoptotic markers, while yca1Delta deleted of YCA1 encoding yeast caspase 1 survived. On the other hand, when cells were exposed to 50 mM VPA, both the wild-type and yca1Delta died showing morphological features similar to those of the autophagic death of cdc28 which was also independent of YCA1. Thus, these results suggested that yeast cells die via YCA1-dependent apoptosis when their proliferative activity is mildly impaired.
丙戊酸（VPA）以剂量依赖的方式抑制酵母生长，在100 mM时达到完全抑制。当细胞暴露于25 mM VPA时，野生型细胞死亡并显示出凋亡标记，而缺失编码酵母半胱天冬酶1的YCA1的yca1Delta细胞存活。另一方面，当细胞暴露于50 mM VPA时，野生型和yca1Delta细胞均死亡，显示出与同样独立于YCA1的cdc28自噬死亡相似的形态特征。因此，这些结果表明，当酵母细胞的增殖活性受到轻度损害时，它们通过YCA1依赖性凋亡死亡。
