Bawadi H A, Bansode R R, Trappey A, Truax R E, Losso J N
Food Protein Biotechnology Laboratory, School of Human Ecology, Louisiana State University Agricultural Center, 111 Food Science Building, Baton Rouge, LA 70803, USA.
Cancer Lett. 2005 Feb 10;218(2):153-62. doi: 10.1016/j.canlet.2004.06.021.
The potential anti-angiogenic activities of water-soluble condensed tannins isolated from black beans were evaluated using HEL 299 normal human fibroblast lung cells, Caco-2 colon, MCF-7 and Hs578T breast, and DU 145 human prostatic cancer cells. Condensed tannins at 0.24-24 microM did not affect the growth of normal cells, but dose-dependently induced cancer cell death by apoptosis as shown by a concentration-dependent decrease in ATP and cell gross morphology. After 24h exposure to Caco-2, MCF-7, Hs578T, and DU 145 cancer cells, water-soluble black bean condensed tannins at 24 microM suppressed fetal bovine serum stimulated cell migration, the secretion of matrix metalloproteinase-2 (MMP-2 or gelatinase A), matrix metalloproteinase-9 (MMP-9 or gelatinase B), and vascular endothelial growth factor VEGF(165) receptor expression by the cancer cells in the conditioned media. The potential health enhancing properties of condensed tannins from black beans as inhibitors of angiogenesis is discussed.
利用HEL 299正常人肺成纤维细胞、Caco - 2结肠癌细胞、MCF - 7和Hs578T乳腺癌细胞以及DU 145人前列腺癌细胞，评估了从黑豆中分离出的水溶性缩合单宁的潜在抗血管生成活性。浓度为0.24 - 24微摩尔的缩合单宁不影响正常细胞的生长，但如ATP浓度依赖性降低和细胞总体形态所示，可通过凋亡剂量依赖性地诱导癌细胞死亡。在将Caco - 2、MCF - 7、Hs578T和DU 145癌细胞暴露于24微摩尔的水溶性黑豆缩合单宁24小时后，其抑制了胎牛血清刺激的细胞迁移、基质金属蛋白酶 - 2（MMP - 2或明胶酶A）、基质金属蛋白酶 - 9（MMP - 9或明胶酶B）的分泌以及条件培养基中癌细胞的血管内皮生长因子VEGF(165)受体表达。本文讨论了黑豆缩合单宁作为血管生成抑制剂的潜在健康促进特性。
