通过靶向基因破坏阐明流感嗜血杆菌Rd细胞壁生物合成途径中的必需基因和非必需基因。
Elucidation of essential and nonessential genes in the Haemophilus influenzae Rd cell wall biosynthetic pathway by targeted gene disruption.
作者信息
Trepod Catherine M, Mott John E
机构信息
Antimicrobials, Pfizer Inc., Groton, CT, USA.
出版信息
Antimicrob Agents Chemother. 2005 Feb;49(2):824-6. doi: 10.1128/AAC.49.2.824-826.2005.
Abstract
Targeted gene disruption by in vitro transposon mutagenesis has been used to identify the genes required for biosynthesis of the Haemophilus influenzae Rd cell wall under standard cultivation conditions. Of the 28 genes known to be associated with the cell wall biosynthetic pathway, 14 were determined to be essential.
摘要
通过体外转座子诱变进行靶向基因破坏，已被用于鉴定在标准培养条件下流感嗜血杆菌Rd细胞壁生物合成所需的基因。在已知与细胞壁生物合成途径相关的28个基因中，有14个被确定为必需基因。
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